Assessment of ochratoxin A occurrence in Argentine red wines using a novel sensitive quechers-solid phase extraction approach prior to ultra high performance liquid chromatography-tandem mass spectrometry methodology.
J Sci Food Agric
; 97(8): 2487-2497, 2017 Jun.
Article
em En
| MEDLINE
| ID: mdl-27701739
BACKGROUND: The assessment of ochratoxin A (OTA) in wine is relevant for food safety and its continuous control allows to reduce the risk of intake. Thus, a novel sensitive QuEChERS-SPE (Quick, Easy, Cheap, Effective, Rugged and Safe - Solid Phase Extraction) pretreatment prior to liquid chromatography coupled to tandem mass spectrometry was developed for the determination of OTA in red wine samples from different grape-growing regions in Argentine. RESULTS: A sensitive methodology was achieved and thus the limits of detection and quantification were 0.02 and 0.05 µg L-1 , respectively. Recoveries ranged from 89.0% to 105.3%. The method was applied to 136 red wine samples (Argentina's flagship varieties: Malbec and Cabernet Sauvignon) from ten grape-growing regions, during vintages 2013-2015. Although all of the samples investigated were contaminated with OTA (concentrations ranged from 0.02 to 0.98 µg L-1 ), the levels detected were lower than the maximum allowable concentration limit of 2.0 µg L-1 established by international regulations. CONCLUSION: The methodology proposed is suitable for reliable OTA analysis in red wines. Similarly, the values obtained from the samples analyzed were in accordance with the current regulations and, as a consequence, preventive actions to reduce this mycotoxin incidence can be undertaken. © 2016 Society of Chemical Industry.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vinho
/
Cromatografia Líquida de Alta Pressão
/
Espectrometria de Massas em Tandem
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Extração em Fase Sólida
/
Micotoxinas
/
Ocratoxinas
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
País/Região como assunto:
America do sul
/
Argentina
Idioma:
En
Revista:
J Sci Food Agric
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Reino Unido