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Iron acquisition and siderophore production in the fish pathogen Renibacterium salmoninarum.
Bethke, J; Poblete-Morales, M; Irgang, R; Yáñez, A; Avendaño-Herrera, R.
Afiliação
  • Bethke J; Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuícola, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Viña del Mar, Chile.
  • Poblete-Morales M; Interdisciplinary Center for Aquaculture Research (INCAR), Concepción, Chile.
  • Irgang R; Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuícola, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Viña del Mar, Chile.
  • Yáñez A; Interdisciplinary Center for Aquaculture Research (INCAR), Concepción, Chile.
  • Avendaño-Herrera R; Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuícola, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Viña del Mar, Chile.
J Fish Dis ; 39(11): 1275-1283, 2016 Nov.
Article em En | MEDLINE | ID: mdl-27696458
Renibacterium salmoninarum is the causative agent of bacterial kidney disease, which significantly affects salmonid farming worldwide. Despite this impact, there is scarce data on its iron uptake ability, a factor of pathogenesis. This study investigated the iron acquisition mechanisms of R. salmoninarum and its capacity to uptake iron from different sources. Thirty-two Chilean isolates and the DSM20767T type strain grew in the presence of 2,2'-Dipyridyl at varying concentrations (250-330 µm), and all isolates positively reacted on chrome azurol S agar. Subsequently, inocula of four Chilean isolates and the type strain were prepared with or without 200 µm of 2,2'-Dipyridyl for uptake assays. Assay results revealed differences between the isolates in terms of iron acquisition. While a prior iron-limited environment was, for most isolates, not required to activate the uptake of iron (II) sulphate, ammonium iron (III) citrate or iron (III) chloride at higher concentrations (100 µm), it did facilitate growth at lower iron concentrations (10 µm and 1 µm). An exception was the H-2 isolate, which only grew with 100 µm of iron sulphide. In turn, 100 µm of haemin was toxic when isolates were grown in normal KDM-2. In silico R. salmoninarumATCC 33209T genome analysis detected various genes coding iron uptake-related proteins. This is the first study indicating two iron acquisition systems in R. salmoninarum: one involving siderophores and another involving haem group utilization. These data represent a first step towards fully elucidating this virulence factor in the pathogenic R. salmoninarum.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Actinomycetales / Sideróforos / Salmo salar / Doenças dos Peixes / Ferro / Micrococcaceae Limite: Animals País/Região como assunto: America do sul / Chile Idioma: En Revista: J Fish Dis Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Actinomycetales / Sideróforos / Salmo salar / Doenças dos Peixes / Ferro / Micrococcaceae Limite: Animals País/Região como assunto: America do sul / Chile Idioma: En Revista: J Fish Dis Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido