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Danger-associated peptide signaling in Arabidopsis requires clathrin.
Ortiz-Morea, Fausto Andres; Savatin, Daniel V; Dejonghe, Wim; Kumar, Rahul; Luo, Yu; Adamowski, Maciej; Van den Begin, Jos; Dressano, Keini; Pereira de Oliveira, Guilherme; Zhao, Xiuyang; Lu, Qing; Madder, Annemieke; Friml, Jirí; Scherer de Moura, Daniel; Russinova, Eugenia.
Afiliação
  • Ortiz-Morea FA; Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; Laboratory of Molecular and Plant Biology, Department of Genetics, College of Agriculture, University of São Paulo, Piracicaba, SP 13418-900, Br
  • Savatin DV; Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium;
  • Dejonghe W; Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium;
  • Kumar R; Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium;
  • Luo Y; Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium;
  • Adamowski M; Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria;
  • Van den Begin J; Laboratory for Organic and Biomimetic Chemistry, Department of Organic Chemistry, Ghent University, 9000 Gent, Belgium.
  • Dressano K; Laboratory of Molecular and Plant Biology, Department of Genetics, College of Agriculture, University of São Paulo, Piracicaba, SP 13418-900, Brazil;
  • Pereira de Oliveira G; Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium;
  • Zhao X; Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium;
  • Lu Q; Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium;
  • Madder A; Laboratory for Organic and Biomimetic Chemistry, Department of Organic Chemistry, Ghent University, 9000 Gent, Belgium.
  • Friml J; Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria;
  • Scherer de Moura D; Laboratory of Molecular and Plant Biology, Department of Genetics, College of Agriculture, University of São Paulo, Piracicaba, SP 13418-900, Brazil;
  • Russinova E; Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; eurus@psb.vib-ugent.be.
Proc Natl Acad Sci U S A ; 113(39): 11028-33, 2016 09 27.
Article em En | MEDLINE | ID: mdl-27651494
The Arabidopsis thaliana endogenous elicitor peptides (AtPeps) are released into the apoplast after cellular damage caused by pathogens or wounding to induce innate immunity by direct binding to the membrane-localized leucine-rich repeat receptor kinases, PEP RECEPTOR1 (PEPR1) and PEPR2. Although the PEPR-mediated signaling components and responses have been studied extensively, the contributions of the subcellular localization and dynamics of the active PEPRs remain largely unknown. We used live-cell imaging of the fluorescently labeled and bioactive pep1 to visualize the intracellular behavior of the PEPRs in the Arabidopsis root meristem. We found that AtPep1 decorated the plasma membrane (PM) in a receptor-dependent manner and cointernalized with PEPRs. Trafficking of the AtPep1-PEPR1 complexes to the vacuole required neither the trans-Golgi network/early endosome (TGN/EE)-localized vacuolar H(+)-ATPase activity nor the function of the brefeldin A-sensitive ADP-ribosylation factor-guanine exchange factors (ARF-GEFs). In addition, AtPep1 and different TGN/EE markers colocalized only rarely, implying that the intracellular route of this receptor-ligand pair is largely independent of the TGN/EE. Inducible overexpression of the Arabidopsis clathrin coat disassembly factor, Auxilin2, which inhibits clathrin-mediated endocytosis (CME), impaired the AtPep1-PEPR1 internalization and compromised AtPep1-mediated responses. Our results show that clathrin function at the PM is required to induce plant defense responses, likely through CME of cell surface-located signaling components.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Transdução de Sinais / Clatrina / Arabidopsis Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2016 Tipo de documento: Article País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Transdução de Sinais / Clatrina / Arabidopsis Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2016 Tipo de documento: Article País de publicação: Estados Unidos