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Investigation of sinonasal microbiome spatial organization in chronic rhinosinusitis.
Ramakrishnan, Vijay R; Gitomer, Sarah; Kofonow, Jennifer M; Robertson, Charles E; Frank, Daniel N.
Afiliação
  • Ramakrishnan VR; Department of Otolaryngology-Head and Neck Surgery, University of Colorado School of Medicine, Denver, CO.
  • Gitomer S; Department of Otolaryngology-Head and Neck Surgery, University of Colorado School of Medicine, Denver, CO.
  • Kofonow JM; Division of Infectious Diseases, University of Colorado School of Medicine, Denver, CO.
  • Robertson CE; Division of Infectious Diseases, University of Colorado School of Medicine, Denver, CO.
  • Frank DN; Microbiome Research Consortium, University of Colorado School of Medicine, Denver, CO.
Int Forum Allergy Rhinol ; 7(1): 16-23, 2017 01.
Article em En | MEDLINE | ID: mdl-27627048
BACKGROUND: Chronic rhinosinusitis (CRS) is a multifactorial inflammatory airway disorder in which bacteria are implicated in the initiation and/or sustenance of disease in some patients. The sinuses are colonized by bacteria even in health, and the potential for sinus-specific niches harboring unique microbial consortia raises questions for clinical and research investigation. The objective was to determine the degree to which resident upper airways microbiota differ between individuals and anatomic sites, in order to determine the optimal site of microbial sampling for study in CRS. METHODS: Eight CRS patients undergoing primary surgery were sampled bilaterally at the anterior nares, middle meatus, nasopharynx, maxillary sinus, frontal sinus, and sphenoid sinus for investigation using broad-range bacterial 16S ribosomal RNA (rRNA) sequencing. RESULTS: Between-subject variability in bacterial microbiota was substantially greater than within-subject variability. The middle meatus was fairly representative of the underlying sinuses, although corynebacteria were detected at higher abundances in the middle meatus, relative to the maxillary (p < 0.1), frontal (p < 0.05), or sphenoid (p < 0.1) sinuses. CONCLUSION: Interpersonal variation of the upper airway microbiome greatly outweighs niche-specific differences. The middle meatus is a fair representation of the underlying sinuses and may be considered for use as a simple single site for sampling in longitudinal studies or in subjects who have not undergone sinus surgery.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Seios Paranasais / Sinusite / Rinite / Microbiota Tipo de estudo: Observational_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Int Forum Allergy Rhinol Ano de publicação: 2017 Tipo de documento: Article País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Seios Paranasais / Sinusite / Rinite / Microbiota Tipo de estudo: Observational_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Int Forum Allergy Rhinol Ano de publicação: 2017 Tipo de documento: Article País de publicação: Estados Unidos