Your browser doesn't support javascript.
loading
Identification of Meliatoxins in Melia azedarach Extracts Using Mass Spectrometry for Quality Control.
Cornelio, Vivian E; Forim, Moacir R; Perlatti, Bruno; Fernandes, João B; Vieira, Paulo C; Napolitano, Michael P; Yost, Richard A; da Silva, Maria Fátima G F.
Afiliação
  • Cornelio VE; Department of Chemistry, Federal University of São Carlos, São Carlos, SP, Brazil.
  • Forim MR; Department of Chemistry, Federal University of São Carlos, São Carlos, SP, Brazil.
  • Perlatti B; Department of Chemistry, Federal University of São Carlos, São Carlos, SP, Brazil.
  • Fernandes JB; Department of Chemistry, Federal University of São Carlos, São Carlos, SP, Brazil.
  • Vieira PC; Department of Chemistry, Federal University of São Carlos, São Carlos, SP, Brazil.
  • Napolitano MP; Department of Chemistry, University of Florida, Gainesville, FL, USA.
  • Yost RA; Department of Chemistry, University of Florida, Gainesville, FL, USA.
  • da Silva MF; Department of Chemistry, Federal University of São Carlos, São Carlos, SP, Brazil.
Planta Med ; 83(3-04): 312-317, 2017 Feb.
Article em En | MEDLINE | ID: mdl-27599262
Indiscriminate use of synthetic pesticides can be hazardous to both humans and the environment, but the use of natural products as a source of bio-based products, such as Melia azedarach extracts, is an interesting approach to overcome these hazards. Unfortunately, the limonoids found in M. azedarach with desired insecticidal properties (e.g. azadirachtin) may also be present with limonoids toxic to mammals. The goal of this report was to develop a fast and reliable MS-based experiment to characterize meliatoxins in crude extracts of M. azedarach, in order to provide unequivocal assessment of the safety for extracts for application in the field. MS and MS/MS experiments using MALDI ionization were evaluated as tools for the assignment of characteristic ions produced by each meliatoxin in crude extracts.The use of different experiments in combination, such as the analysis of fragment m/z 557 and [M + Na]+ (adducts ions m/z 681 and m/z 667), MALDI-MS can be used for detection of meliatoxins A1/B1 or A2/B2 in a crude extract and may be used to discriminate meliatoxins A from B, respectively. Subsequent MS/MS experiments can distinguish between the presence of group 1 and/or 2 in each class of meliatoxins classifying the proposed approach as a quick and efficient quality control method of meliatoxins in real M. azedarach samples.
Assuntos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Melia azedarach / Limoninas Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Planta Med Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Melia azedarach / Limoninas Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Planta Med Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha