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Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients.
Chomiski, Verônica; Gragnani, Alfredo; Bonucci, Jéssica; Correa, Silvana Aparecida Alves; Noronha, Samuel Marcos Ribeiro de; Ferreira, Lydia Masako.
Afiliação
  • Chomiski V; Fellow MSc degree, Division of Plastic Surgery, Department of Surgery, Universidade Federal de São Paulo (UNIFESP0, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, technical procedures, manuscript writing.
  • Gragnani A; PhD, Associate Professor, Division of Plastic Surgery, Department of Surgery, UNIFESP, Sao Paulo-SP, Brazil. Conception, design, intellectual and scientific content of the study; analysis and interpretation of data; manuscript writing; critical revision.
  • Bonucci J; Fellow MSc degree, Division of Plastic Surgery, Department of Surgery, UNIFESP, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, technical procedures.
  • Correa SA; PhD, Postdoctoral degree, Division of Plastic Surgery, Department of Surgery, UNIFESP, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, technical procedures, acquisition of data, manuscript writing.
  • Noronha SM; PhD, Postdoctoral degree, Division of Plastic Surgery, Department of Surgery, UNIFESP, Sao Paulo-SP, Brazil. Acquisition and interpretation of data, manuscript writing.
  • Ferreira LM; Head, Full Professor, Division of Plastic Surgery, UNIFESP, Researcher 1A-CNPq, Director Medicine III-CAPES, Sao Paulo-SP, Brazil. Intellectual and scientific content of the study.
Acta Cir Bras ; 31(8): 505-12, 2016 Aug.
Article em En | MEDLINE | ID: mdl-27579877
PURPOSE: To evaluate the effect of keratinocyte growth factor (KGF) treatment on the expression of wound-healing-related genes in cultured keratinocytes from burn patients. METHODS: Keratinocytes were cultured and divided into 4 groups (n=4 in each group): TKB (KGF-treated keratinocytes from burn patients), UKB (untreated keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls), and UKC (untreated keratinocytes from controls). Gene expression analysis using quantitative polymerase chain reaction (qPCR) array was performed to compare (1) TKC versus UKC, (2) UKB versus UKC, (3) TKB versus UKC, (4) TKB versus UKB, (5) TKB versus TKC, and (6) UKB versus TKC. RESULTS: Comparison 1 showed one down-regulated and one up-regulated gene; comparisons 2 and 3 resulted in the same five down-regulated genes; comparison 4 had no significant difference in relative gene expression; comparison 5 showed 26 down-regulated and 7 up-regulated genes; and comparison 6 showed 25 down-regulated and 11 up-regulated genes. CONCLUSION: There was no differential expression of wound-healing-related genes in cultured primary keratinocytes from burn patients treated with keratinocyte growth factor.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cicatrização / Queimaduras / Expressão Gênica / Queratinócitos / Fator 7 de Crescimento de Fibroblastos Tipo de estudo: Observational_studies Limite: Adult / Animals / Female / Humans / Male Idioma: En Revista: Acta Cir Bras Ano de publicação: 2016 Tipo de documento: Article País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cicatrização / Queimaduras / Expressão Gênica / Queratinócitos / Fator 7 de Crescimento de Fibroblastos Tipo de estudo: Observational_studies Limite: Adult / Animals / Female / Humans / Male Idioma: En Revista: Acta Cir Bras Ano de publicação: 2016 Tipo de documento: Article País de publicação: Brasil