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Antioxidant Capacity of Gallic Acid in vitro Assayed on Human Erythrocytes.
Suwalsky, Mario; Colina, José; Gallardo, María José; Jemiola-Rzeminska, Malgorzata; Strzalka, Kazimierz; Manrique-Moreno, Marcela; Sepúlveda, Benjamín.
Afiliação
  • Suwalsky M; Faculty of Chemical Sciences, University of Concepción, Concepción, Chile. msuwalsk@udec.cl.
  • Colina J; Faculty of Chemical Sciences, University of Concepción, Concepción, Chile.
  • Gallardo MJ; Center for Optics and Photonics, University of Concepción, Concepción, Chile.
  • Jemiola-Rzeminska M; Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
  • Strzalka K; Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
  • Manrique-Moreno M; Faculty of Exact and Natural Sciences, University of Antioquia, A.A. 1226, Medellín, Colombia.
  • Sepúlveda B; Center for Optics and Photonics, University of Concepción, Concepción, Chile.
J Membr Biol ; 249(6): 769-779, 2016 12.
Article em En | MEDLINE | ID: mdl-27568391
Gallic acid (GA) is a polyphenol present in many plants. This study was aimed to investigate the molecular interaction of GA with the human erythrocyte membrane and to determine its antioxidant capacity. The molecular interaction with the membrane of human red cells and the antioxidant property was assayed on both human red cells and molecular models of its membrane. Observations by optical, scanning electron, and defocusing microscopy demonstrated that GA is capable to convert red cells from their normal biconcave shape to crenated echinocytes. This result indicates that GA molecules are positioned in the outer monolayer of the red cell membrane. Dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE) were selected as classes of phospholipids found in the outer and inner monolayers of the red cell membrane, respectively. X-ray diffraction and differential scanning calorimetry showed that GA was preferentially bound to DMPC bilayers. Experiments related to the antioxidant capacity of GA indicated that this compound offsets HClO oxidative capacity on DMPE bilayers. In addition, optical, scanning, defocusing microscopy, and hemolysis assays confirmed the protective capacity of GA against HClO deleterious effects on human red cells. As a conclusion, GA would be capable to block the access of oxidants into the lipid bilayer, and thus avoid their access into red cells.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Eritrócitos / Ácido Gálico / Antioxidantes Limite: Humans Idioma: En Revista: J Membr Biol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Eritrócitos / Ácido Gálico / Antioxidantes Limite: Humans Idioma: En Revista: J Membr Biol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos