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Rhizobium hidalgonense sp. nov., a nodule endophytic bacterium of Phaseolus vulgaris in acid soil.
Yan, Jun; Yan, Hui; Liu, Li Xue; Chen, Wen Feng; Zhang, Xiao Xia; Verástegui-Valdés, Myrthala M; Wang, En Tao; Han, Xiao Zeng.
Afiliação
  • Yan J; Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, People's Republic of China.
  • Yan H; State Key Laboratory for Agro-Biotechnology, Beijing, 100193, People's Republic of China.
  • Liu LX; Rhizobium Research Center and College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
  • Chen WF; State Key Laboratory for Agro-Biotechnology, Beijing, 100193, People's Republic of China.
  • Zhang XX; Rhizobium Research Center and College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
  • Verástegui-Valdés MM; State Key Laboratory for Agro-Biotechnology, Beijing, 100193, People's Republic of China.
  • Wang ET; Rhizobium Research Center and College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
  • Han XZ; Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
Arch Microbiol ; 199(1): 97-104, 2017 Jan.
Article em En | MEDLINE | ID: mdl-27557842
One Gram-negative, aerobic, motile, rod-shaped bacterium, designated as FH14T, was isolated from nodules of Phaseolus vulgaris grown in Hidalgo State of Mexico. Results based upon 16S rRNA gene (≥99.8 % similarities to known species), concatenated sequence (recA, atpD and glnII) analysis of three housekeeping genes (≤93.4 % similarities to known species) and average nucleotide identity (ANI) values of genome sequence (ranged from 87.6 to 90.0 % to related species) indicated the distinct position of strain FH14T within the genus Rhizobium. In analyses of symbiotic genes, only nitrogen fixation gene nifH was amplified that had nucleotide sequence identical to those of the bean-nodulating strains in R. phaseoli and R. vallis, while nodulation gene nodC gene was not amplified. The failure of nodulation to its original host P. vulgaris and other legumes evidenced the loss of its nodulation capability. Strain FH14T contained summed feature 8 (C18:1 ω6c/C18:1 ω7c, 59.96 %), C16:0 (10.6 %) and summed feature 2 (C12:0 aldehyde/unknown 10.928, 10.24 %) as the major components of cellular fatty acids. Failure to utilize alaninamide, and utilizing L-alanine, L-asparagine and γ-amino butyric acid as carbon source, distinguished the strain FH14T from the type strains for the related species. The genome size and DNA G+C content of FH14T were 6.94 Mbp and 60.8 mol %, respectively. Based on those results, a novel specie in Rhizobium, named Rhizobium hidalgonense sp. nov., was proposed, with FH14T (=HAMBI 3636T = LMG 29288T) as the type strain.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rhizobium / Microbiologia do Solo / Phaseolus / Nódulos Radiculares de Plantas / Endófitos País/Região como assunto: Mexico Idioma: En Revista: Arch Microbiol Ano de publicação: 2017 Tipo de documento: Article País de publicação: Alemanha
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rhizobium / Microbiologia do Solo / Phaseolus / Nódulos Radiculares de Plantas / Endófitos País/Região como assunto: Mexico Idioma: En Revista: Arch Microbiol Ano de publicação: 2017 Tipo de documento: Article País de publicação: Alemanha