Potential biocontrol actinobacteria: Rhizospheric isolates from the Argentine Pampas lowlands legumes.

Solans, Mariana; Scervino, Jose Martin; Messuti, María Inés; Vobis, Gernot; Wall, Luis Gabriel

Solans, Mariana; Scervino, Jose Martin; Messuti, María Inés; Vobis, Gernot; Wall, Luis Gabriel.

Afiliação

Solans M; INIBIOMA, UNComahue, CONICET. CRUB, Quintral 1250, S. C. Bariloche, 8400, Argentina.
Scervino JM; INIBIOMA, UNComahue, CONICET. CRUB, Quintral 1250, S. C. Bariloche, 8400, Argentina.
Messuti MI; INIBIOMA, UNComahue, CONICET. CRUB, Quintral 1250, S. C. Bariloche, 8400, Argentina.
Vobis G; INIBIOMA, UNComahue, CONICET. CRUB, Quintral 1250, S. C. Bariloche, 8400, Argentina.
Wall LG; Departamento de Ciencia y Tecnología, Programa de Interacciones Biológicas, UNQuilmes, CONICET, R. Saénz Peña 352, B1876BXD Bernal, Argentina.

J Basic Microbiol ; 56(11): 1289-1298, 2016 Nov.

Article em En | MEDLINE | ID: mdl-27550574

RESUMO

Control of fungal plant diseases by using naturally occurring non-pathogenic microorganisms represents a promising approach to biocontrol agents. This study reports the isolation, characterization, and fungal antagonistic activity of actinobacteria from forage soils in the Flooding Pampa, Argentina. A total of 32 saprophytic strains of actinobacteria were obtained by different isolation methods from rhizospheric soil of Lotus tenuis growing in the Salado River Basin. Based on physiological traits, eight isolates were selected for their biocontrol-related activities such as production of lytic extracellular enzymes, siderophores, hydrogen cyanide (HCN), and antagonistic activity against Cercospora sojina, Macrophomia phaseolina, Phomopsis sp., Fusarium oxysporum, and Fusarium verticilloides. These actinobacteria strains were characterized morphologically, physiologically, and identified by using molecular techniques. The characterization of biocontrol-related activities in vitro showed positive results for exoprotease, phospholipase, fungal growth inhibition, and siderophore production. However, none of the strains was positive for the production of hydrogen cyanide (HCN). Streptomyces sp. MM140 presented the highest index for biocontrol, and appear to be promising pathogenic fungi biocontrol agents. These results show the potential capacity of actinobacteria isolated from forage soils in the Argentine Pampas lowlands as promising biocontrol agents, and their future agronomic applications.

Assuntos

Actinobacteria/isolamento & purificação; Actinobacteria/fisiologia; Antibiose; Agentes de Controle Biológico/isolamento & purificação; Fabaceae/microbiologia; Rizosfera; Microbiologia do Solo; Actinobacteria/química; Actinobacteria/genética; Antifúngicos/farmacologia; Argentina; Ascomicetos; Produtos Biológicos/farmacologia; Fusarium/efeitos dos fármacos; Cianeto de Hidrogênio/metabolismo; Doenças das Plantas/microbiologia; Sideróforos/biossíntese; Streptomyces/isolamento & purificação

Palavras-chave

Actinobacteria; Antagonistic activity; Biocontrol; Potential agronomic applications

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microbiologia do Solo / Actinobacteria / Rizosfera / Agentes de Controle Biológico / Fabaceae / Antibiose País/Região como assunto: America do sul / Argentina Idioma: En Revista: J Basic Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Argentina País de publicação: Alemanha

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