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Integrated analysis of miRNA and mRNA gene expression microarrays: Influence on platelet reactivity, clopidogrel response and drug-induced toxicity.
Freitas, Renata Caroline Costa de; Bortolin, Raul Hernandes; Lopes, Mariana Borges; Hirata, Mario Hiroyuki; Hirata, Rosario Dominguez Crespo; Silbiger, Vivian Nogueira; Luchessi, André Ducati.
Afiliação
  • Freitas RCC; Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
  • Bortolin RH; Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
  • Lopes MB; Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
  • Hirata MH; School of Pharmaceutical Sciences, University of Sao Paulo, São Paulo, Brazil.
  • Hirata RDC; School of Pharmaceutical Sciences, University of Sao Paulo, São Paulo, Brazil.
  • Silbiger VN; Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
  • Luchessi AD; Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil. Electronic address: andre.luchessi@outlook.com.
Gene ; 593(1): 172-178, 2016 Nov 15.
Article em En | MEDLINE | ID: mdl-27543010
Genetic and epigenetic variability may influence the efficacy and safety of antiplatelet therapies, including clopidogrel. Therefore, the miRNA-mRNA interactions and drug toxicity were investigated in silico using available microarray data. Expressions profiles of platelet miRNA (GSE59488) from acute coronary syndrome and mRNA in peripheral blood cells (GSE32226) from coronary artery disease patients were used to miRNA-target mRNA integrated analysis by Ingenuity Pathways Analysis 6 software (IPA). Results showed that ST13 mRNA is regulated by hsa-miR-107 (miR-103-3p); BTNL3 and CFD mRNAs are regulated by hsa-miR-4701-3p (miR-1262); SLC7A8 is regulated by hsa-miR-145-5p (miR-145-5p); and SENP5 mRNA is regulated by hsa-miR-15b-5p (miR-16-5p) and hsa-miR-26a-5p (miR-26a-5p). Drug toxicity IPA tool showed that these miRNAs/mRNAs are associated with clopidogrel-related liver and renal injury. In conclusion, these results demonstrate that differential expression of miRNAs in platelets and interactions with their target mRNAs are associated with variability in platelet reactivity, clopidogrel response and drug-induced toxicity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plaquetas / Ticlopidina / RNA Mensageiro / Regulação da Expressão Gênica / Bases de Dados de Ácidos Nucleicos / MicroRNAs Limite: Animals / Humans Idioma: En Revista: Gene Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plaquetas / Ticlopidina / RNA Mensageiro / Regulação da Expressão Gênica / Bases de Dados de Ácidos Nucleicos / MicroRNAs Limite: Animals / Humans Idioma: En Revista: Gene Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda