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Discovery of clubroot-resistant genes in Brassica napus by transcriptome sequencing.
Chen, S W; Liu, T; Gao, Y; Zhang, C; Peng, S D; Bai, M B; Li, S J; Xu, L; Zhou, X Y; Lin, L B.
Afiliação
  • Chen SW; College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
  • Liu T; College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
  • Gao Y; College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
  • Zhang C; College of Tropical Crops, Yunnan Agricultural University, Kunming, China.
  • Peng SD; Agricultural Experimental Teaching Center, Yunnan Agricultural University, Kunming, China.
  • Bai MB; College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
  • Li SJ; College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
  • Xu L; College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
  • Zhou XY; College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China.
  • Lin LB; College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, China linliangbin-63@163.com.
Genet Mol Res ; 15(3)2016 Aug 12.
Article em En | MEDLINE | ID: mdl-27525940
Clubroot significantly affects plants of the Brassicaceae family and is one of the main diseases causing serious losses in B. napus yield. Few studies have investigated the clubroot-resistance mechanism in B. napus. Identification of clubroot-resistant genes may be used in clubroot-resistant breeding, as well as to elucidate the molecular mechanism behind B. napus clubroot-resistance. We used three B. napus transcriptome samples to construct a transcriptome sequencing library by using Illumina HiSeq™ 2000 sequencing and bioinformatic analysis. In total, 171 million high-quality reads were obtained, containing 96,149 unigenes of N50-value. We aligned the obtained unigenes with the Nr, Swiss-Prot, clusters of orthologous groups, and gene ontology databases and annotated their functions. In the Kyoto encyclopedia of genes and genomes database, 25,033 unigenes (26.04%) were assigned to 124 pathways. Many genes, including broad-spectrum disease-resistance genes, specific clubroot-resistant genes, and genes related to indole-3-acetic acid (IAA) signal transduction, cytokinin synthesis, and myrosinase synthesis in the Huashuang 3 variety of B. napus were found to be related to clubroot-resistance. The effective clubroot-resistance observed in this variety may be due to the induced increased expression of these disease-resistant genes and strong inhibition of the IAA signal transduction, cytokinin synthesis, and myrosinase synthesis. The homology observed between unigenes 0048482, 0061770 and the Crr1 gene shared 94% nucleotide similarity. Furthermore, unigene 0061770 could have originated from an inversion of the Crr1 5'-end sequence.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Brassica napus / Resistência à Doença Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Brassica napus / Resistência à Doença Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China País de publicação: Brasil