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Liposome-entrapped GABA modulates the expression of nNOS in NG108-15 cells.
Vaz, Gisele C; Sharma, Neeru M; Zheng, Hong; Zimmerman, Matthew C; Santos, Robson S; Frezard, Frederic; Fontes, Marco A P; Patel, Kaushik P.
Afiliação
  • Vaz GC; Department of Physiology & Biophysics, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Sharma NM; Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE 68198-5850, United States.
  • Zheng H; Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE 68198-5850, United States.
  • Zimmerman MC; Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE 68198-5850, United States.
  • Santos RS; Department of Physiology & Biophysics, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Frezard F; Department of Physiology & Biophysics, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Fontes MAP; Department of Physiology & Biophysics, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Patel KP; Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE 68198-5850, United States. Electronic address: kpatel@unmc.edu.
J Neurosci Methods ; 273: 55-63, 2016 11 01.
Article em En | MEDLINE | ID: mdl-27523033
BACKGROUND: Liposomes are concentric lipid vesicles that allow a sustained release of entrapped substances. GABA (γ-aminobutyric acid) is the most prevalent inhibitory neurotransmitter in the central nervous system. NEW METHOD: Using GABA-containing liposomes (GL) prepared by the freeze-thawing method, we determined the effect of sustained release of GABA on expression of neuronal nitric oxide synthase (nNOS) and GABAA receptor (GABAAR) in an in vitro neuronal model. RESULTS: Neuronal cell line NG108-15 treated with different doses of GL during 24h showed an increase in expression of GABAAR (54 and 50% with 10 and 20ng doses, respectively) and nNOS (138, 157 and 165% with 20, 50 and 100ng doses, respectively) compared with cells treated with empty liposomes (EL). Additionally, cells treated with 50ng of GL showed an increase in GABAAR (23%) after 1h followed by an increase in nNOS (55, 46 and 55%) at 8, 12 and 24h time points, respectively. Immunofluorescence experiments confirmed an increase in nNOS (134%) and basal intracellular levels of nitric oxide (84%) after GL treatment. Further, treatment of cells with GL showed a decrease in expression of a protein inhibitor of nNOS (PIN) (26, 66 and 57% with 20, 50 and 100ng doses respectively) compared with control. COMPARISON WITH EXISTING METHODS: This is first demonstration for the development of GL that allows sustained slow release of this neurotransmitter. CONCLUSION: These results suggest that a slow release of GABA can change the expression of nNOS possibly via alteration in PIN levels in neuronal cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulação Neoplásica da Expressão Gênica / Óxido Nítrico Sintase Tipo I / Ácido gama-Aminobutírico / Lipossomos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Neurosci Methods Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulação Neoplásica da Expressão Gênica / Óxido Nítrico Sintase Tipo I / Ácido gama-Aminobutírico / Lipossomos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Neurosci Methods Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda