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Galanin subtype 1 and subtype 2 receptors mediate opposite anxiety-like effects in the rat dorsal raphe nucleus.
Morais, J S; Souza, M M; Campanha, T M N; Muller, C J T; Bittencourt, A S; Bortoli, V C; Schenberg, L C; Beijamini, V.
Afiliação
  • Morais JS; Biochemistry and Pharmacology Graduation Program, Federal University of Espirito Santo, Brazil.
  • Souza MM; Department of Pharmaceutical Sciences, Health Science Center, Federal University of Espirito Santo, Brazil.
  • Campanha TM; Department of Pharmaceutical Sciences, Health Science Center, Federal University of Espirito Santo, Brazil.
  • Muller CJ; Physiological Sciences Graduation Program, Federal University of Espirito Santo, Brazil.
  • Bittencourt AS; Biochemistry and Pharmacology Graduation Program, Federal University of Espirito Santo, Brazil.
  • Bortoli VC; Biochemistry and Pharmacology Graduation Program, Federal University of Espirito Santo, Brazil.
  • Schenberg LC; Biochemistry and Pharmacology Graduation Program, Federal University of Espirito Santo, Brazil; Physiological Sciences Graduation Program, Federal University of Espirito Santo, Brazil.
  • Beijamini V; Biochemistry and Pharmacology Graduation Program, Federal University of Espirito Santo, Brazil; Department of Pharmaceutical Sciences, Health Science Center, Federal University of Espirito Santo, Brazil. Electronic address: vanessabh77@gmail.com.
Behav Brain Res ; 314: 125-33, 2016 11 01.
Article em En | MEDLINE | ID: mdl-27498247
About 40% of the dorsal raphe nucleus (DRN) neurons co-express serotonin (5-HT) and galanin. Serotonergic pathways from the DRN to the amygdala facilitate learned anxiety, while those from the DRN to the dorsal periaqueductal grey matter (DPAG) impair innate anxiety. Previously, we showed that galanin infusion in the DRN of rats induces anxiolytic effect by impairing inhibitory avoidance without changing escape behaviour in the elevated T-maze (ETM). Here, we evaluated: (1) which galanin receptors would be involved in the anxiolytic effect of galanin in the DRN of rats tested in the ETM; (2) the effects of galanin intra-DRN on panic-like behaviours evoked by electrical stimulation of the DPAG. The activation of DRN GAL1 receptors by M617 (1.0 and 3.0nmol) facilitated inhibitory avoidance, whereas the activation of GAL2 receptors by AR-M1896 (3.0nmol) impaired the inhibitory avoidance in the ETM, suggesting an anxiogenic and an anxiolytic-like effect respectively. Both agonists did not change escape behaviour in the ETM or locomotor activity in the open field. The anxiolytic effect of AR-M1896 was attenuated by the prior administration of WAY100635 (0.18nmol), a 5-HT1A antagonist. Galanin (0.3nmol) administered in the DRN increased discreetly flight behaviours induced by electrical stimulation of the DPAG, suggesting a panicolytic effect. Together, our results showed that galanin mediates opposite anxiety responses in the DRN by activation of GAL1 and GAL2 receptors. The anxiolytic effect induced by activation of Gal2 receptors may depend on serotonergic tone. Finally, the role of galanin in panic related behaviours remains uncertain.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ansiedade / Galanina / Receptor Tipo 1 de Galanina / Receptor Tipo 2 de Galanina / Núcleo Dorsal da Rafe Limite: Animals Idioma: En Revista: Behav Brain Res Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ansiedade / Galanina / Receptor Tipo 1 de Galanina / Receptor Tipo 2 de Galanina / Núcleo Dorsal da Rafe Limite: Animals Idioma: En Revista: Behav Brain Res Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda