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HLA-F coding and regulatory segments variability determined by massively parallel sequencing procedures in a Brazilian population sample.
Lima, Thálitta Hetamaro Ayala; Buttura, Renato Vidal; Donadi, Eduardo Antônio; Veiga-Castelli, Luciana Caricati; Mendes-Junior, Celso Teixeira; Castelli, Erick C.
Afiliação
  • Lima THA; Molecular Genetics and Bioinformatics Laboratory, Experimental Research Unit (UNIPEX), Sector 5, School of Medicine, Unesp - Univ. Estadual Paulista, Botucatu, State of São Paulo, Brazil.
  • Buttura RV; Molecular Genetics and Bioinformatics Laboratory, Experimental Research Unit (UNIPEX), Sector 5, School of Medicine, Unesp - Univ. Estadual Paulista, Botucatu, State of São Paulo, Brazil.
  • Donadi EA; School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, State of São Paulo, Brazil.
  • Veiga-Castelli LC; School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, State of São Paulo, Brazil.
  • Mendes-Junior CT; Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
  • Castelli EC; Molecular Genetics and Bioinformatics Laboratory, Experimental Research Unit (UNIPEX), Sector 5, School of Medicine, Unesp - Univ. Estadual Paulista, Botucatu, State of São Paulo, Brazil; Pathology Department, School of Medicine, Unesp - Univ. Estadual Paulista, Botucatu, State of São Paulo, Brazil.
Hum Immunol ; 77(10): 841-853, 2016 Oct.
Article em En | MEDLINE | ID: mdl-27448841
Human Leucocyte Antigen F (HLA-F) is a non-classical HLA class I gene distinguished from its classical counterparts by low allelic polymorphism and distinctive expression patterns. Its exact function remains unknown. It is believed that HLA-F has tolerogenic and immune modulatory properties. Currently, there is little information regarding the HLA-F allelic variation among human populations and the available studies have evaluated only a fraction of the HLA-F gene segment and/or have searched for known alleles only. Here we present a strategy to evaluate the complete HLA-F variability including its 5' upstream, coding and 3' downstream segments by using massively parallel sequencing procedures. HLA-F variability was surveyed on 196 individuals from the Brazilian Southeast. The results indicate that the HLA-F gene is indeed conserved at the protein level, where thirty coding haplotypes or coding alleles were detected, encoding only four different HLA-F full-length protein molecules. Moreover, a same protein molecule is encoded by 82.45% of all coding alleles detected in this Brazilian population sample. However, the HLA-F nucleotide and haplotype variability is much higher than our current knowledge both in Brazilians and considering the 1000 Genomes Project data. This protein conservation is probably a consequence of the key role of HLA-F in the immune system physiology.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antígenos de Histocompatibilidade Classe I / Sequências Reguladoras de Ácido Nucleico / Fases de Leitura Aberta / Sistema Imunitário Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Hum Immunol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antígenos de Histocompatibilidade Classe I / Sequências Reguladoras de Ácido Nucleico / Fases de Leitura Aberta / Sistema Imunitário Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Hum Immunol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos