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First Detection of Toxoplasma gondii DNA in the Fresh Leafs of Vegetables in South America.
Marchioro, Ariella A; Tiyo, Bruna T; Colli, Cristiane M; de Souza, Carla Z; Garcia, João L; Gomes, Mônica L; Falavigna-Guilherme, Ana L.
Afiliação
  • Marchioro AA; 1 Environmental and Food Parasitology Laboratory, State University of Maringá (UEM) , Maringá, Brazil .
  • Tiyo BT; 1 Environmental and Food Parasitology Laboratory, State University of Maringá (UEM) , Maringá, Brazil .
  • Colli CM; 1 Environmental and Food Parasitology Laboratory, State University of Maringá (UEM) , Maringá, Brazil .
  • de Souza CZ; 1 Environmental and Food Parasitology Laboratory, State University of Maringá (UEM) , Maringá, Brazil .
  • Garcia JL; 2 Department of Veterinary Medicine, State University of Londrina , Londrina, Brazil .
  • Gomes ML; 1 Environmental and Food Parasitology Laboratory, State University of Maringá (UEM) , Maringá, Brazil .
  • Falavigna-Guilherme AL; 1 Environmental and Food Parasitology Laboratory, State University of Maringá (UEM) , Maringá, Brazil .
Vector Borne Zoonotic Dis ; 16(9): 624-6, 2016 09.
Article em En | MEDLINE | ID: mdl-27421086
Despite the high dispersion of Toxoplasma gondii oocysts in the environment, there are few studies investigating their presence in vegetables consumed by the general population. This has led us to investigate its occurrence in raw vegetables. A total of 238 samples of vegetables were collected, including crisp lettuce, regular lettuce, chicory, rocket, and parsley, both organic and nonorganic, locally in northwestern Parana, Southern Brazil. Each sample (50 g) was washed and filtered separately. A PCR was performed to detect the parasite DNA from the sediment of each sample, using B1 (B22-23) and Toxo4-5 primers. We found contamination in 3.8% of the samples, 0.8% with the primer Toxo4-5 and 2.9% with B22-B23. The results were positive in 0.6% (1/62) of the samples of smooth lettuce, 3.7% (4/106) of crisp head lettuce, 5.0% (2/40) of chicory, 14.3% (1/7) of rocket, and 20% (1/5) of parsley. These data show the contamination by T. gondii in raw vegetables directly from production sites and stores, in both organic and nonorganic samples.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Toxoplasma / Verduras / DNA de Protozoário / Parasitologia de Alimentos / Folhas de Planta Tipo de estudo: Diagnostic_studies Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Vector Borne Zoonotic Dis Assunto da revista: DOENCAS TRANSMISSIVEIS / EPIDEMIOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Toxoplasma / Verduras / DNA de Protozoário / Parasitologia de Alimentos / Folhas de Planta Tipo de estudo: Diagnostic_studies Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Vector Borne Zoonotic Dis Assunto da revista: DOENCAS TRANSMISSIVEIS / EPIDEMIOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos