Bioethanol production by reusable Saccharomyces cerevisiae immobilized in a macroporous monolithic hydrogel matrices.
J Biotechnol
; 233: 56-65, 2016 Sep 10.
Article
em En
| MEDLINE
| ID: mdl-27396938
Performance of yeasts on industrial processes can be dramatically improved by immobilization of the biocatalyst. The immobilization of Saccharomyces cerevisiae inside monolithic macroporous hydrogels were produced by in-situ polymerization of acrylamide around a live yeast suspension under cryogelation conditions. Preculture of the yeasts was not necessary and this innovative and simple procedure is amenable to scaling-up to industrial production. The yeasts were efficiently retained in monolithic hydrogels, presenting excellent mechanical properties and high cell viability. Macroporous hydrogels showed a fast mass transport allowing the hydrogel-yeast complexes achieved similar ethanol yield and productivity than free yeasts, which is larger than those reached with yeasts immobilized in compact hydrogels. Moreover, the same yeasts were able to maintain its activity by up to five reaction cycles with a cell single batch during fermentation reactions.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Células Imobilizadas
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Reatores Biológicos
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Hidrogel de Polietilenoglicol-Dimetacrilato
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Etanol
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Biocombustíveis
Idioma:
En
Revista:
J Biotechnol
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Holanda