Your browser doesn't support javascript.
loading
High-Throughput Sequencing Reveals Immunological Characteristics of the TRB-/IgH-CDR3 Region of Umbilical Cord Blood.
Guo, Changlong; Wang, Qidi; Cao, Xiaofang; Yang, Ying; Liu, Xin; An, Lisha; Cai, Ruikun; Du, Meng; Wang, Guangyu; Qiu, Yue; Peng, Zuoqi; Han, Jian; Ni, Shuhua; Tan, Xuerui; Jin, Li; Yu, Song; Wang, Huiying; Wang, Chunlin; Wang, Xingyu; Ma, Xu.
Afiliação
  • Guo C; Department of Genetics, National Research Institute for Family Planning, Beijing, China.
  • Wang Q; Department of Genetics, National Research Institute for Family Planning, Beijing, China.
  • Cao X; Department of Genetics, National Research Institute for Family Planning, Beijing, China. Electronic address: mybrother1984@163.com.
  • Yang Y; Department of Genetics, National Research Institute for Family Planning, Beijing, China.
  • Liu X; Department of Genetics, National Research Institute for Family Planning, Beijing, China; Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing, China.
  • An L; Department of Genetics, National Research Institute for Family Planning, Beijing, China.
  • Cai R; Department of Genetics, National Research Institute for Family Planning, Beijing, China.
  • Du M; Department of Genetics, National Research Institute for Family Planning, Beijing, China; Graduate School of Peking Union Medical College, Beijing, China.
  • Wang G; Department of Genetics, National Research Institute for Family Planning, Beijing, China.
  • Qiu Y; Department of Genetics, National Research Institute for Family Planning, Beijing, China; Graduate School of Peking Union Medical College, Beijing, China.
  • Peng Z; Department of Genetics, National Research Institute for Family Planning, Beijing, China.
  • Han J; HudsonAlpha Institute for Biotechnology, Huntsville, AL.
  • Ni S; First Affiliated Hospital, Medical College of Shantou University, Shantou, Guangdong, China.
  • Tan X; First Affiliated Hospital, Medical College of Shantou University, Shantou, Guangdong, China.
  • Jin L; Beijing Friendship Hospital, Capital Medical University, Beijing, China.
  • Yu S; Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Healthy Care Hospital, Beijing, China.
  • Wang H; Beijing Shijitan Hospital, Capital Medical University, Beijing, China.
  • Wang C; Department of Genetics, National Research Institute for Family Planning, Beijing, China; Stanford Genome Technology Center, Stanford University, Palo Alto, CA.
  • Wang X; Department of Genetics, National Research Institute for Family Planning, Beijing, China; Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing, China.
  • Ma X; Department of Genetics, National Research Institute for Family Planning, Beijing, China; Graduate School of Peking Union Medical College, Beijing, China.
J Pediatr ; 176: 69-78.e1, 2016 09.
Article em En | MEDLINE | ID: mdl-27373756
OBJECTIVE: To compare the differences of immunological characteristics between newborn and adults, we performed high-throughput sequencing to reveal the diversity of umbilical cord blood and adult peripheral blood at both T-cell receptor beta chain (TRB) and immunoglobulin heavy chain (IGH) levels. STUDY DESIGN: High-throughput sequencing was performed to analyze the expression of TRB-CDR3 and IGH-CDR3 in circulating T and B cells isolated from 20 healthy adults, 56 pregnant women, and 40 newborns. RESULTS: Our results revealed different immunological characteristics between newborn and adults, such as distinctive complementarity determining region 3 (CDR3) lengths, usage bias of variable and joining segments, random nucleotide addition, a large number of unique CDR3 peptides, and a greater repertoire diversity. Moreover, each newborn had a distinctive TRB-/IGH-CDR3 repertoire that was independent of the maternal immune status. CONCLUSIONS: This study presents comprehensive, unrestricted profiles of the TRB/IGH-CDR3 repertoire of newborns, pregnant women, and healthy adults at a sequence-level resolution. Our data may contribute to a better understanding of the immune system of newborns and benefit the efficient application of umbilical cord blood transplantation in future.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores de Antígenos de Linfócitos T alfa-beta / Cadeias Pesadas de Imunoglobulinas / Análise de Sequência de DNA / Regiões Determinantes de Complementaridade / Sangue Fetal / Sequenciamento de Nucleotídeos em Larga Escala Limite: Adult / Female / Humans / Newborn / Pregnancy Idioma: En Revista: J Pediatr Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores de Antígenos de Linfócitos T alfa-beta / Cadeias Pesadas de Imunoglobulinas / Análise de Sequência de DNA / Regiões Determinantes de Complementaridade / Sangue Fetal / Sequenciamento de Nucleotídeos em Larga Escala Limite: Adult / Female / Humans / Newborn / Pregnancy Idioma: En Revista: J Pediatr Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos