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Tissue-selective inflammation in the oral cavity of the rat.
Frade, Taíssa Iolanda Checón; Dos Reis, Diego Carlos; Cassali, Geovanni Dantas; Bakhle, Yeshwant S; de Francischi, Janetti Nogueira.
Afiliação
  • Frade TI; Dentistry Faculty at Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
  • Dos Reis DC; General Pathology Department of Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
  • Cassali GD; General Pathology Department of Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
  • Bakhle YS; Leukocyte Biology, NHLI, Imperial College, London, UK.
  • de Francischi JN; Pharmacology Department of Biological Sciences Institute (ICB), Federal University of Minas Gerais, Av. Antonio Carlos 6627, Pampulha, Belo Horizonte, MG, Brazil. janettif@icb.ufmg.br.
Inflammopharmacology ; 24(4): 145-53, 2016 Aug.
Article em En | MEDLINE | ID: mdl-27324249
In the current study, carrageenan (CG; 100-1000 µg/site) was injected intraorally in the cheeks of Holtzman or Wistar rats to evaluate the consequences of administration of a non-immunogenic stimulus in the orofacial region. Subsequent inflammation was measured as oedema (increased thickness of the cheek wall using digital calipers), relative to the other cheek injected with saline. Oedema formation and tissue collection for histopathological studies were assessed at 0.5, 1, 2, 3, 4, 6, 24, 48, 72, 96, 120 and 144 h after injection. In parallel, other groups of rats were injected with CG in the hind paw, to provide a reference response. The inhibitor of prostaglandin biosynthesis, indomethacin, and antagonists of histamine, serotonin and NK1 receptors were injected s.c., 0.5 h before CG. CG induced a dose-related oedema more rapidly from 0 to 2 h which lasted for at least 72 h, showing a biphasic profile (peak at 2 and 24 h), compared with the monophasic oedema induced in rat paws (maximal duration of 24 h). Histopathological analysis of the CG-injected cheek revealed oedema formation with little leukocyte recruitment at 1-3 h, mast cell degranulation at 6 h, and a mixed polymorphonuclear and mononuclear cell infiltrate by 24 h. Histamine and serotonin antagonists and indomethacin, but not the NK1 antagonist, decreased cheek oedema in the first 4 h following carrageenan. Taken together, our data indicated important differences in the pattern of inflammation between the oral cavity and the paw which will determine the therapeutic approach to the treatment of inflammatory conditions in the oral cavity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carragenina / Modelos Animais de Doenças / Edema / Boca Limite: Animals Idioma: En Revista: Inflammopharmacology Assunto da revista: FARMACOLOGIA / TERAPIA POR MEDICAMENTOS Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carragenina / Modelos Animais de Doenças / Edema / Boca Limite: Animals Idioma: En Revista: Inflammopharmacology Assunto da revista: FARMACOLOGIA / TERAPIA POR MEDICAMENTOS Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça