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In vitro effect of dexmedetomidine on the respiratory burst of neutrophils.
Chen, S L; Zhou, W; Hua, F Z; Chen, Y; Zheng, X L; Long, X F; Lu, J.
Afiliação
  • Chen SL; Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.
  • Zhou W; Department of Vascular Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.
  • Hua FZ; Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.
  • Chen Y; Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.
  • Zheng XL; Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.
  • Long XF; Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.
  • Lu J; Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.
Genet Mol Res ; 15(2)2016 Jun 03.
Article em En | MEDLINE | ID: mdl-27323135
The immunosuppressive effects of dexmedetomidine, a highly selective and widely used a2-adrenoceptor agonist for sedation, analgesia, and stress management, are investigated in vitro. In the present study, the respiratory burst of human neutrophils separated from venous blood was evaluated with dexmedetomidine treatment after Escherichia coli stimulation. The effects of five concentrations of dexmedetomidine (1, 5, 10, 50, 100 µg/mL) were evaluated by rhodamine in a flow cytometer. The nitric oxide (NO) production and nitric oxide synthase (iNOS) activity were also determined by using commercial kits. The results were compared to the positive control responses (respiratory burst without drug). We found that dexmedetomidine significantly suppressed respiratory burst, NO production, and iNOS activity after stimulation with E. coli, in a dose-dependent manner. The suppressive effects of dexmedetomidine on phagocytic activity of human neutrophils were associated with respiratory burst coupled with NO production.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Explosão Respiratória / Dexmedetomidina / Óxido Nítrico Sintase Tipo II / Imunossupressores / Neutrófilos Limite: Humans Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Explosão Respiratória / Dexmedetomidina / Óxido Nítrico Sintase Tipo II / Imunossupressores / Neutrófilos Limite: Humans Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China País de publicação: Brasil