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Exploring the effects of low-level laser therapy on fibroblasts and tumor cells following gamma radiation exposure.
Ramos Silva, Camila; Cabral, Fernanda Viana; de Camargo, Claudinei Francisco Morais; Núñez, Silvia Cristina; Mateus Yoshimura, Tania; de Lima Luna, Arthur Cássio; Maria, Durvanei Augusto; Ribeiro, Martha Simões.
Afiliação
  • Ramos Silva C; Nuclear Technology Postgraduate Program, University of Sao Paulo, Av. Prof. Lineu Prestes, 2242, 05508-000, São Paulo, Brazil.
  • Cabral FV; Laboratory of Optical Therapy (Center for Lasers and Applications/Nuclear and Energy Research Institute), Av. Prof. Lineu Prestes, 2242, 05508-000, Brazil.
  • de Camargo CF; Nuclear Technology Postgraduate Program, University of Sao Paulo, Av. Prof. Lineu Prestes, 2242, 05508-000, São Paulo, Brazil.
  • Núñez SC; Laboratory of Optical Therapy (Center for Lasers and Applications/Nuclear and Energy Research Institute), Av. Prof. Lineu Prestes, 2242, 05508-000, Brazil.
  • Mateus Yoshimura T; Nuclear Technology Postgraduate Program, University of Sao Paulo, Av. Prof. Lineu Prestes, 2242, 05508-000, São Paulo, Brazil.
  • de Lima Luna AC; Laboratory of Optical Therapy (Center for Lasers and Applications/Nuclear and Energy Research Institute), Av. Prof. Lineu Prestes, 2242, 05508-000, Brazil.
  • Maria DA; Laboratory of Optical Therapy (Center for Lasers and Applications/Nuclear and Energy Research Institute), Av. Prof. Lineu Prestes, 2242, 05508-000, Brazil.
  • Ribeiro MS; Nuclear Technology Postgraduate Program, University of Sao Paulo, Av. Prof. Lineu Prestes, 2242, 05508-000, São Paulo, Brazil.
J Biophotonics ; 9(11-12): 1157-1166, 2016 12.
Article em En | MEDLINE | ID: mdl-27322660
Ionizing radiation (IR) induces DNA damage and low-level laser therapy (LLLT) has been investigated to prevent or repair detrimental outcomes resulting from IR exposure. Few in vitro studies, however, explore the biological mechanisms underlying those LLLT benefits. Thus, in this work, fibroblasts and tumor cells are submitted to IR with doses of 2.5 Gy and 10 Gy. After twenty-four-h, the cells are exposed to LLLT with fluences of 30 J cm-2 , 90 J cm-2 , and 150 J cm-2 . Cellular viability, cell cycle phases, cell proliferation index and senescence are evaluated on days 1 and 4 after LLLT irradiation. For fibroblasts, LLLT promotes - in a fluence-dependent manner - increments in cell viability and proliferation, while a reduction in the senescence was observed. Regarding tumor cells, no influences of LLLT on cell viability are noticed. Whereas LLLT enhances cell populations in S and G2 /M cell cycle phases for both cellular lines, a decrease in proliferation and increase in senescence was verified only for tumor cells. Putting together, the results suggest that fibroblasts and tumor cells present different responses to LLLT following exposure to gamma-radiation, and these promising results should stimulate further investigations. Senescence of tumor cells and fibroblasts on the 4th day after ionizing radiation (IR) and low-level laser therapy (LLLT) exposures. The number of senescent cells increased significantly for tumor cells (a) while for fibroblasts no increment was observed (b). The blue collor indicates senescence activity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Senescência Celular / Exposição à Radiação / Terapia com Luz de Baixa Intensidade / Fibroblastos / Raios gama Limite: Humans Idioma: En Revista: J Biophotonics Assunto da revista: BIOFISICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Senescência Celular / Exposição à Radiação / Terapia com Luz de Baixa Intensidade / Fibroblastos / Raios gama Limite: Humans Idioma: En Revista: J Biophotonics Assunto da revista: BIOFISICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha