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Production of cyclodextrin glycosyltransferase by immobilized Bacillus sp. on chitosan matrix.
Es, Ismail; Ribeiro, Maycon Carvalho; Dos Santos Júnior, Samuel Rodrigues; Khaneghah, Amin Mousavi; Rodriguez, Armando Garcia; Amaral, André Corrêa.
Afiliação
  • Es I; Molecular Biology and Genetics, Institute of Biological Science, Federal University of Goiás, Goiânia, GO, Brazil.
  • Ribeiro MC; Molecular Biology and Genetics, Institute of Biological Science, Federal University of Goiás, Goiânia, GO, Brazil.
  • Dos Santos Júnior SR; Department of Biotechnology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil.
  • Khaneghah AM; Department of Food Science, Faculty of Food Engineering, University of Campinas, Campinas, São Paulo, Brazil.
  • Rodriguez AG; Department of Biochemistry and Molecular Biology, Institute of Biological Science, Federal University of Goiás, Goiânia, GO, Brazil.
  • Amaral AC; Department of Biotechnology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil. amaral.nanobiotech@gmail.com.
Bioprocess Biosyst Eng ; 39(10): 1487-500, 2016 Oct.
Article em En | MEDLINE | ID: mdl-27194141
The whole-cell immobilization on chitosan matrix was evaluated. Bacillus sp., as producer of CGTase, was grown in solid-state and batch cultivation using three types of starches (cassava, potato and cornstarch). Biomass growth and substrate consumption were assessed by flow cytometry and modified phenol-sulfuric acid assays, respectively. Qualitative analysis of CGTase production was determined by colorless area formation on solid culture containing phenolphthalein. Scanning electron microscopy (SEM) analysis demonstrated that bacterial cells were immobilized on chitosan matrix efficiently. Free cells reached very high numbers during batch culture while immobilized cells maintained initial inoculum concentration. The maximum enzyme activity achieved by free cells was 58.15 U ml(-1) (36 h), 47.50 U ml(-1) (36 h) and 68.36 U ml(-1) (36 h) on cassava, potato and cornstarch, respectively. CGTase activities for immobilized cells were 82.15 U ml(-1) (18 h) on cassava, 79.17 U ml(-1) (12 h) on potato and 55.37 U ml(-1) (in 6 h and max 77.75 U ml(-1) in 36 h) on cornstarch. Application of immobilization technique increased CGTase activity significantly. The immobilized cells produced CGTase with higher activity in a shorter fermentation time comparing to free cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus / Proteínas de Bactérias / Quitosana / Glucosiltransferases Tipo de estudo: Qualitative_research Idioma: En Revista: Bioprocess Biosyst Eng Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus / Proteínas de Bactérias / Quitosana / Glucosiltransferases Tipo de estudo: Qualitative_research Idioma: En Revista: Bioprocess Biosyst Eng Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha