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Optimization of Trichomonas vaginalis Diagnosis during Pregnancy at a University Hospital, Argentina.
Testardini, Pamela; Vaulet, María Lucía Gallo; Entrocassi, Andrea Carolina; Menghi, Claudia; Eliseht, Martha Cora; Gatta, Claudia; Losada, Mirta; Touzón, María Sol; Corominas, Ana; Vay, Carlos; Tatti, Silvio; Famiglietti, Angela; Fermepin, Marcelo Rodriguez; Perazzi, Beatriz.
Afiliação
  • Testardini P; Clinical Bacteriology Laboratory, Department of Clinical Biochemistry, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Vaulet ML; Clinical Immmunology and Virology Laboratory, Department of Clinical Biochemistry, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Entrocassi AC; Clinical Immmunology and Virology Laboratory, Department of Clinical Biochemistry, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Menghi C; Clinical Immmunology and Virology Laboratory, Department of Clinical Biochemistry, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Eliseht MC; Clinical Parasitology Laboratory, Department of Clinical Biochemistry, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Gatta C; Lower Genital Tract, Obstetrics Division, Department of Tocogynecology, Hospital de Clinicas, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Losada M; Clinical Parasitology Laboratory, Department of Clinical Biochemistry, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Touzón MS; Clinical Bacteriology Laboratory, Department of Clinical Biochemistry, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Corominas A; Clinical Bacteriology Laboratory, Department of Clinical Biochemistry, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Vay C; Clinical Immmunology and Virology Laboratory, Department of Clinical Biochemistry, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Tatti S; Clinical Bacteriology Laboratory, Department of Clinical Biochemistry, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Famiglietti A; Lower Genital Tract, Obstetrics Division, Department of Tocogynecology, Hospital de Clinicas, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Fermepin MR; Clinical Bacteriology Laboratory, Department of Clinical Biochemistry, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Perazzi B; Clinical Immmunology and Virology Laboratory, Department of Clinical Biochemistry, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Korean J Parasitol ; 54(2): 191-5, 2016 Apr.
Article em En | MEDLINE | ID: mdl-27180578
The aim of this study was to evaluate different methods for Trichomonas vaginalis diagnosis during pregnancy in order to prevent maternal and perinatal complications. A total of 386 vaginal exudates from pregnant women were analyzed. T. vaginalis was investigated by 3 types of microscopic examinations direct wet mount with physiologic saline solution, prolonged May-Grunwald Giemsa (MGG) staining, and wet mount with sodium-acetate-formalin (SAF)/methylene blue method. PCR for 18S rRNA gene as well as culture in liquid medium were performed. The sensitivity and specificity of the microscopic examinations were evaluated considering the culture media positivity or the PCR techniques as gold standard. The frequency of T. vaginalis infection was 6.2% by culture and/or PCR, 5.2% by PCR, 4.7% by culture, 3.1% by SAF/methylene blue method and 2.8% by direct wet smear and prolonged MGG staining. The sensitivities were 83.3%, 75.0%, 50.0%, and 45.8% for PCR, culture, SAF/methylene blue method, and direct wet smear-prolonged MGG staining, respectively. The specificity was 100% for all the assessed methods. Microscopic examinations showed low sensitivity, mainly in asymptomatic pregnant patients. It is necessary to improve the detection of T. vaginalis using combined methods providing higher sensitivity, such as culture and PCR, mainly in asymptomatic pregnant patients, in order to prevent maternal and perinatal complications.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Coloração e Rotulagem / Trichomonas vaginalis / Vaginite por Trichomonas / Reação em Cadeia da Polimerase Tipo de estudo: Diagnostic_studies Limite: Female / Humans / Pregnancy País/Região como assunto: America do sul / Argentina Idioma: En Revista: Korean J Parasitol Assunto da revista: PARASITOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Argentina País de publicação:
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Coloração e Rotulagem / Trichomonas vaginalis / Vaginite por Trichomonas / Reação em Cadeia da Polimerase Tipo de estudo: Diagnostic_studies Limite: Female / Humans / Pregnancy País/Região como assunto: America do sul / Argentina Idioma: En Revista: Korean J Parasitol Assunto da revista: PARASITOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Argentina País de publicação: