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Clonidine-stimulated growth hormone concentrations (cut-off values) measured by immunochemiluminescent assay (ICMA) in children and adolescents with short stature.
Borges, Maria de Fátima; Teixeira, Flávia Carolina Cândida; Feltrin, Aline Karin; Ribeiro, Karina Alvarenga; Nascentes, Gabriel Antonio Nogueira; Resende, Elisabete Aparecida Mantovani Rodrigues; Ferreira, Beatriz Pires; Silva, Adriana Paula; Palhares, Heloísa Marcelina Cunha.
Afiliação
  • Borges Mde F; Unidade de Endocrinologia Pediátrica, Divisão de Endocrinologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil.
  • Teixeira FC; Unidade de Endocrinologia Pediátrica, Divisão de Endocrinologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil.
  • Feltrin AK; Unidade de Endocrinologia Pediátrica, Divisão de Endocrinologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil.
  • Ribeiro KA; Unidade de Endocrinologia Pediátrica, Divisão de Endocrinologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil.
  • Nascentes GA; Microbiologia e Imunologia, Instituto Federal do Triângulo Mineiro, Uberaba, MG, Brazil.
  • Resende EA; Unidade de Endocrinologia Pediátrica, Divisão de Endocrinologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil.
  • Ferreira BP; Unidade de Endocrinologia Pediátrica, Divisão de Endocrinologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil.
  • Silva AP; Unidade de Endocrinologia Pediátrica, Divisão de Endocrinologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil.
  • Palhares HM; Unidade de Endocrinologia Pediátrica, Divisão de Endocrinologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil.
Clinics (Sao Paulo) ; 71(4): 226-31, 2016 Apr.
Article em En | MEDLINE | ID: mdl-27166774
OBJECTIVES: To establish cut-off values for growth hormone concentrations using clonidine as a secretagogue and an immunochemiluminescent assay as the method of measurement and to analyze the response time as well as the influence of gender, nutritional status and pubertal stage. METHODS: A total of 225 tests were performed in 3 patient groups, categorized as group 1 (normal), group 2 (idiopathic short stature) and group 3 (growth hormone deficiency). Among the 199 disease-free individuals, 138 were prepubertal, and 61 were pubertal. Clonidine (0.1 mg/m2) was orally administered, and the growth hormone level was measured by immunochemiluminescent assay. The growth hormone peak and the difference between the growth hormone peak and the baseline level were then analyzed. Statistical analyses were performed using Student's t-test or the Mann-Whitney test and Kruskal-Wallis test followed by Dunn's post hoc test. Cut-off values were determined using a receiver operating characteristic curve. RESULTS: Group 1 and group 2 had no difference in growth hormone peak, gender, body mass index standard deviation score, or pubertal stage. Group 3 exhibited a significantly lower growth hormone peak than the other groups did. The receiver operating characteristic curve demonstrated that growth hormone concentrations ≥ 3.0 ng/mL defined responsiveness to clonidine. In total, 3.02% of individuals in group 1 and group 2 were considered false positive, i.e., these children lacked growth hormone deficiency and had a peak below 3.0 ng/mL. CONCLUSION: Clonidine-stimulated growth hormone concentrations ≥3 ng/mL, as measured by immunochemiluminescent assay, suggest responsiveness to the stimulus regardless of gender, body mass index standard deviation score or pubertal stage.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estatura / Hormônio do Crescimento / Clonidina / Hormônio do Crescimento Humano / Agonistas de Receptores Adrenérgicos alfa 2 / Transtornos do Crescimento Tipo de estudo: Etiology_studies / Observational_studies / Prognostic_studies Limite: Adolescent / Child / Child, preschool / Female / Humans / Male Idioma: En Revista: Clinics (Sao Paulo) Assunto da revista: MEDICINA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estatura / Hormônio do Crescimento / Clonidina / Hormônio do Crescimento Humano / Agonistas de Receptores Adrenérgicos alfa 2 / Transtornos do Crescimento Tipo de estudo: Etiology_studies / Observational_studies / Prognostic_studies Limite: Adolescent / Child / Child, preschool / Female / Humans / Male Idioma: En Revista: Clinics (Sao Paulo) Assunto da revista: MEDICINA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos