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The systemic and gonadal toxicity of 3-methylcholanthrene is prevented by daily administration of α-naphthoflavone.
Rhon-Calderón, Eric Alejandro; Galarza, Rocío Alejandra; Lomniczi, Alejandro; Faletti, Alicia Graciela.
Afiliação
  • Rhon-Calderón EA; Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Galarza RA; Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Lomniczi A; Division of Neuroscience, Oregon National Primate Research Center, Beaverton, OR 97006, USA.
  • Faletti AG; Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina. Electronic address: alifaletti@gmail.com.
Toxicology ; 353-354: 58-69, 2016 04 15.
Article em En | MEDLINE | ID: mdl-27163632
In the present study, we investigated the effect of 3-methylcholanthrene (3MC) on sexual maturity and the ability of α-naphthoflavone (αNF) to prevent this action. To this end, immature rats were daily injected intraperitoneally with 3MC (0.1 or 1mg/kg) and/or αNF (80mg/kg). Body weight, vaginal opening and estrous cycle were recorded and ovaries were obtained on the day of estrus. Ovarian weight, ovulation rate (measured by the number of oocytes within oviducts), and follicular development (determined by histology) were studied. No differences were found in body weight, ovarian weight, day of vaginal opening, or the establishment of the estrous cycle among the different groups of rats. However, animals treated with 3MC, at both doses, exhibited a lower number of primordial, primary, preantral and antral follicles than controls. Also, 3MC inhibited the ovulation rate and induced an overexpression of both the Cyp1a1 and Cyp1b1 genes, measured by chromatin immunoprecipitation assay. The daily treatment with αNF alone increased the number of follicles in most of the stages analyzed when compared with controls. Moreover, the αNF treatment prevented completely not only the 3MC-induced decrease in all types of follicles but also the 3MC-induced overexpression of Cyp enzymes and the genetic damage in bone marrow cells and oocytes. These results suggest that (i) daily exposure to 3MC during the pubertal period destroys the follicle reserve and alters the ovulation rate; (ii) the 3MC action seems to be mediated by an aryl hydrocarbon receptor-dependent mechanism; (iii) daily administration of αNF has a clear stimulatory action on the ovarian function; and (iv) αNF may prevent both the systemic and gonadal 3MC-induced toxicity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Benzoflavonas / Citocromo P-450 CYP1A1 / Citocromo P-450 CYP1B1 / Metilcolantreno Limite: Animals Idioma: En Revista: Toxicology Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Argentina País de publicação: Irlanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Benzoflavonas / Citocromo P-450 CYP1A1 / Citocromo P-450 CYP1B1 / Metilcolantreno Limite: Animals Idioma: En Revista: Toxicology Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Argentina País de publicação: Irlanda