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Short hairpin rna targeting insulin-like growth fator binding protein-3 restores the bioavailability of insulin-like growth factor-1 in diabetic rats.
Zhou, Zhang-Yan; Zhong, Guang-Jun; Cheng, Shao-Ping; Huang, Hui; Wang, Jing; Pan, Hui; Liu, Chang-Mao; Xing, Cheng; Sun, Ya-Ling; Liu, Rong-Hua; Li, Fei.
Afiliação
  • Zhou ZY; Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
  • Zhong GJ; Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
  • Cheng SP; Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
  • Huang H; Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
  • Wang J; Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
  • Pan H; Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
  • Liu CM; Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
  • Xing C; Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
  • Sun YL; Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
  • Liu RH; Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
  • Li F; Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, HuBei, China.
Int Braz J Urol ; 42(1): 139-45, 2016.
Article em En | MEDLINE | ID: mdl-27136480
PURPOSE: To investigate whether intracavernosal injection of short hairpin RNA for IGFBP-3 could improve erectile function in streptozotocin-induced diabetic rats. MATERIALS AND METHODS: After 12 weeks of IGFBP-3 short hairpin RNA injection treatment, intracavernous pressure responses to electrical stimulation of cavernous nerves were evaluated. The expression of IGFBP-3 and IGF-1 at mRNA and protein levels were detected by quantitative real-time PCR analysis and Western blot, respectively. The concentration of cavernous cyclic guanosine monophosphate was detected by enzyme-linked immunosorbent assay. RESULTS: At 12 weeks after intracavernous administration of IGFBP-3 shRNA, the cavernosal pressure was significantly increased in response to the cavernous nerves stimulation compared to the diabetic group (P<0.05). Cavernous IGFBP-3 expression at both mRNA and protein levels was significantly inhibited. At the same time, cavernous IGF-1 expression was significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic group (P<0.01). Cavernous cyclic guanosine monophosphate concentration was significantly increased in the IGFBP-3 shRNA treatment group compared to the diabetic group (P<0.01). CONCLUSIONS: Gene transfer of IGFBP-3 shRNA could improve erectile function via the restoration of cavernous IGF-1 bioavailability and an increase of cavernous cGMP concentration in the pathogenesis of erectile dysfunction in streptozotocin-induced diabetic rats.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pênis / Fator de Crescimento Insulin-Like I / Proteína 3 de Ligação a Fator de Crescimento Semelhante à Insulina / RNA Interferente Pequeno / Diabetes Mellitus Experimental / Disfunção Erétil Tipo de estudo: Etiology_studies / Evaluation_studies Limite: Animals Idioma: En Revista: Int Braz J Urol Assunto da revista: UROLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pênis / Fator de Crescimento Insulin-Like I / Proteína 3 de Ligação a Fator de Crescimento Semelhante à Insulina / RNA Interferente Pequeno / Diabetes Mellitus Experimental / Disfunção Erétil Tipo de estudo: Etiology_studies / Evaluation_studies Limite: Animals Idioma: En Revista: Int Braz J Urol Assunto da revista: UROLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China País de publicação: Brasil