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High Frequency of Chlamydia trachomatis Mixed Infections Detected by Microarray Assay in South American Samples.
Gallo Vaulet, Lucía; Entrocassi, Carolina; Portu, Ana I; Castro, Erica; Di Bartolomeo, Susana; Ruettger, Anke; Sachse, Konrad; Rodriguez Fermepin, Marcelo.
Afiliação
  • Gallo Vaulet L; Universidad de Buenos Aires. Cátedra de Microbiología Clínica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
  • Entrocassi C; Universidad de Buenos Aires. Cátedra de Microbiología Clínica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
  • Portu AI; Universidad de Buenos Aires. Cátedra de Microbiología Clínica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
  • Castro E; Facultad de Medicina, Universidad de San Sebastián, Concepción, Chile.
  • Di Bartolomeo S; Sección Bioquímica Microbiológica, Servicio de Bioquímica, Hospital Nacional Profesor A. Posadas, Buenos Aires, Argentina.
  • Ruettger A; Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Jena, Germany.
  • Sachse K; Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Jena, Germany.
  • Rodriguez Fermepin M; Universidad de Buenos Aires. Cátedra de Microbiología Clínica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
PLoS One ; 11(4): e0153511, 2016.
Article em En | MEDLINE | ID: mdl-27082962
Chlamydia trachomatis is one of the most common sexually transmitted infections worldwide. Based on sequence variation in the ompA gene encoding the major outer membrane protein, the genotyping scheme distinguishes 17 recognized genotypes, i.e. A, B, Ba, C, D, Da, E, F, G, H, I, Ia, J, K, L1, L2, and L3. Genotyping is an important tool for epidemiological tracking of C. trachomatis infections, including the revelation of transmission pathways and association with tissue tropism and pathogenicity. Moreover, genotyping can be useful for clinicians to establish the correct treatment when LGV strains are detected. Recently a microarray assay was described that offers several advantages, such as rapidity, ease of standardization and detection of mixed infections. The aim of this study was to evaluate the performance of the DNA microarray-based assay for C. trachomatis genotyping of clinical samples already typed by PCR-RFLP from South America. The agreement between both typing techniques was 90.05% and the overall genotype distribution obtained with both techniques was similar. Detection of mixed-genotype infections was significantly higher using the microarray assay (8.4% of cases) compared to PCR-RFLP (0.5%). Among 178 samples, the microarray assay identified 10 ompA genotypes, i.e. D, Da, E, F, G, H, I, J, K and L2. The most predominant type was genotype E, followed by D and F.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Chlamydia / Chlamydia trachomatis / Análise de Sequência com Séries de Oligonucleotídeos / Técnicas de Diagnóstico Molecular Tipo de estudo: Diagnostic_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Female / Humans / Male País/Região como assunto: America do sul Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Chlamydia / Chlamydia trachomatis / Análise de Sequência com Séries de Oligonucleotídeos / Técnicas de Diagnóstico Molecular Tipo de estudo: Diagnostic_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Female / Humans / Male País/Região como assunto: America do sul Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos