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Topical anti-inflammatory phytomedicine based on Sphagneticola trilobata dried extracts.
Fucina, Giovana; Rocha, Lilian Wünsch; da Silva, Gislaine Francieli; Hoepers, Silmara Mendes; Ferreira, Fernanda Peres; Guaratini, Thais; Cechinel Filho, Valdir; Lucinda-Silva, Ruth Meri; Quintão, Nara Lins Meira; Bresolin, Tania Mari Bellé.
Afiliação
  • Fucina G; a Graduate Program in Pharmaceutical Sciences , University of Itajaí Valley (UNIVALI) , Itajaí , SC , Brazil.
  • Rocha LW; a Graduate Program in Pharmaceutical Sciences , University of Itajaí Valley (UNIVALI) , Itajaí , SC , Brazil.
  • da Silva GF; a Graduate Program in Pharmaceutical Sciences , University of Itajaí Valley (UNIVALI) , Itajaí , SC , Brazil.
  • Hoepers SM; a Graduate Program in Pharmaceutical Sciences , University of Itajaí Valley (UNIVALI) , Itajaí , SC , Brazil.
  • Ferreira FP; c Lychnoflora Pesquisa e Desenvolvimento em Produtos Naturais LTDA , Ribeirão Preto , SP , Brazil.
  • Guaratini T; c Lychnoflora Pesquisa e Desenvolvimento em Produtos Naturais LTDA , Ribeirão Preto , SP , Brazil.
  • Cechinel Filho V; d NPPNS, Faculty of Pharmaceutical Sciences of Ribeirão Preto , University of São Paulo , Ribeirão Preto São Paulo , Brazil.
  • Lucinda-Silva RM; a Graduate Program in Pharmaceutical Sciences , University of Itajaí Valley (UNIVALI) , Itajaí , SC , Brazil.
  • Quintão NL; b Núcleo de Investigações Químico-Farmacêuticas (NIQFAR) - Pharmacy Course , University of Itajaí Valley (UNIVALI) , Itajaí , SC , Brazil.
  • Bresolin TM; a Graduate Program in Pharmaceutical Sciences , University of Itajaí Valley (UNIVALI) , Itajaí , SC , Brazil.
Pharm Biol ; 54(11): 2465-2474, 2016 Nov.
Article em En | MEDLINE | ID: mdl-27046333
CONTEXT: The aerial parts of Sphagneticola trilobata (L.) Pruski (Asteraceae) are popularly used to treat topical inflammation, but have not been fully investigated. OBJECTIVE: To identify polar compounds in S. trilobata extracts and develop a new topical phytomedicine based on the kaurenoic acid (KA) content while monitoring and demonstrating its topical anti-inflammatory activity. MATERIALS AND METHODS: Ethanol spray-dried extract of S. trilobata was analysed by LC-MS while the KA content from semisolid was analysed by LC-UV. The extent of ear edema induced by applying 20 µL of croton oil (2.5%), arachidonic acid (AA; 2 mg/ear) and decanoylphorbol-13-acetate (TPA; 2.5 mg/ear) in mice was used to evaluate the biological activity of the semisolids, which were applied 30 min before the phlogistic agents. RESULTS: Eight phenylpropanoids and four oleanane-type triterpenoid saponins were identified, majority of them reported for the first time in this species, in addition to KA. The semisolid containing 1.0% of dried extract reduced the ear edema induced by croton oil [77.2 ± 4.5%; ID50 = 0.49 (0.28-0.87%)], TPA (81.5 ± 2.4%) and AA (39.1 ± 6.9%), with decreasing effect at higher KA concentrations. This was accompanied by neutrophil migration inhibition as investigated by biochemical and histological assays. DISCUSSION AND CONCLUSION: The anti-inflammatory effects were (at least in part) due to the interference in protein kinase C (PKC) activation, AA-cascade products and neutrophil migration inhibition, demonstrating the efficacy of the folk topical usage of this plant. The results support the development of a novel topical anti-inflammatory phytomedicine properly standardized to treat inflammatory dermatological diseases.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Asteraceae / Fitoterapia / Anti-Inflamatórios Limite: Animals Idioma: En Revista: Pharm Biol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Asteraceae / Fitoterapia / Anti-Inflamatórios Limite: Animals Idioma: En Revista: Pharm Biol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido