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Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium.
Muñiz-Márquez, Diana B; Contreras, Juan C; Rodríguez, Raúl; Mussatto, Solange I; Teixeira, José A; Aguilar, Cristóbal N.
Afiliação
  • Muñiz-Márquez DB; Group of Bioprocesses, Food Research Department, School of Chemistry, University Autonomous of Coahuila, 25280 Saltillo, Coahuila, Mexico.
  • Contreras JC; Group of Bioprocesses, Food Research Department, School of Chemistry, University Autonomous of Coahuila, 25280 Saltillo, Coahuila, Mexico.
  • Rodríguez R; Group of Bioprocesses, Food Research Department, School of Chemistry, University Autonomous of Coahuila, 25280 Saltillo, Coahuila, Mexico.
  • Mussatto SI; Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, Netherlands.
  • Teixeira JA; IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal.
  • Aguilar CN; Group of Bioprocesses, Food Research Department, School of Chemistry, University Autonomous of Coahuila, 25280 Saltillo, Coahuila, Mexico. Electronic address: cristobal.aguilar@uadec.edu.mx.
Bioresour Technol ; 213: 276-282, 2016 Aug.
Article em En | MEDLINE | ID: mdl-27036329
The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347U/L) was obtained at 32°C, using packing density of 0.7g/cm(3). Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Aspergillus oryzae / Biotecnologia / Hexosiltransferases Idioma: En Revista: Bioresour Technol Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Aspergillus oryzae / Biotecnologia / Hexosiltransferases Idioma: En Revista: Bioresour Technol Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido