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Microwave assisted direct saponification for the simultaneous determination of cholesterol and cholesterol oxides in shrimp.
Souza, Hugo A L; Mariutti, Lilian R B; Bragagnolo, Neura.
Afiliação
  • Souza HAL; Department of Food Science, Faculty of Food Engineering, University of Campinas, 13083-862 São Paulo, Brazil.
  • Mariutti LRB; Department of Food Science, Faculty of Food Engineering, University of Campinas, 13083-862 São Paulo, Brazil.
  • Bragagnolo N; Department of Food Science, Faculty of Food Engineering, University of Campinas, 13083-862 São Paulo, Brazil. Electronic address: neurabragagnolo@gmail.com.
J Steroid Biochem Mol Biol ; 169: 88-95, 2017 05.
Article em En | MEDLINE | ID: mdl-27013019
A novel microwave-assisted direct saponification method for the simultaneous determination of cholesterol and cholesterol oxides in shrimp was developed and validated. Optimal saponification conditions, determined by means of an experimental design, were achieved using 500mg of sample and 20mL of 1mol/L KOH ethanol solution for 16min at 45°C at maximum power at 200W and magnetic stirring at 120rpm. Higher extraction of cholesterol oxides in a reduced saponification time (∼75 times) was achieved in comparison with the direct cold saponification method. The new method showed low detection (≤0.57µg/mL) and quantification (≤1.73µg/mL) limits, good repeatability (≤10.50% intraday and ≤8.56% interday) and low artifact formation (evaluated by using a deuterated cholesterol-D6 standard). Raw, salted and dried-salted shrimps were successfully analyzed by the validated method. The content of cholesterol oxides increased after salting and decreased after drying.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óxidos / Colesterol / Crustáceos / Análise de Alimentos / Micro-Ondas Limite: Animals Idioma: En Revista: J Steroid Biochem Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óxidos / Colesterol / Crustáceos / Análise de Alimentos / Micro-Ondas Limite: Animals Idioma: En Revista: J Steroid Biochem Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido