Microwave assisted direct saponification for the simultaneous determination of cholesterol and cholesterol oxides in shrimp.
J Steroid Biochem Mol Biol
; 169: 88-95, 2017 05.
Article
em En
| MEDLINE
| ID: mdl-27013019
A novel microwave-assisted direct saponification method for the simultaneous determination of cholesterol and cholesterol oxides in shrimp was developed and validated. Optimal saponification conditions, determined by means of an experimental design, were achieved using 500mg of sample and 20mL of 1mol/L KOH ethanol solution for 16min at 45°C at maximum power at 200W and magnetic stirring at 120rpm. Higher extraction of cholesterol oxides in a reduced saponification time (â¼75 times) was achieved in comparison with the direct cold saponification method. The new method showed low detection (≤0.57µg/mL) and quantification (≤1.73µg/mL) limits, good repeatability (≤10.50% intraday and ≤8.56% interday) and low artifact formation (evaluated by using a deuterated cholesterol-D6 standard). Raw, salted and dried-salted shrimps were successfully analyzed by the validated method. The content of cholesterol oxides increased after salting and decreased after drying.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Óxidos
/
Colesterol
/
Crustáceos
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Análise de Alimentos
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Micro-Ondas
Limite:
Animals
Idioma:
En
Revista:
J Steroid Biochem Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Reino Unido