Immunohistochemical expression of GLUT-1, GLUT-3, and carbonic anhydrase IX in benign odontogenic lesions.

Vasconcelos, Roseane Carvalho; de Oliveira Moura, Jamile Marinho Bezerra; Lacerda Brasileiro Junior, Vilson; da Silveira, Éricka Janine Dantas; de Souza, Lélia Batista

Vasconcelos, Roseane Carvalho; de Oliveira Moura, Jamile Marinho Bezerra; Lacerda Brasileiro Junior, Vilson; da Silveira, Éricka Janine Dantas; de Souza, Lélia Batista.

Afiliação

Vasconcelos RC; Postgraduate Program in Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
de Oliveira Moura JM; Postgraduate Program in Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Lacerda Brasileiro Junior V; Postgraduate Program in Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
da Silveira ÉJ; Postgraduate Program in Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
de Souza LB; Postgraduate Program in Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil. leliabsouza@gmail.com, r.roseane@hotmail.com.

J Oral Pathol Med ; 45(9): 712-717, 2016 Oct.

Article em En | MEDLINE | ID: mdl-27009762

RESUMO

BACKGROUND: Some benign odontogenic lesions have a distinct biological behavior with high recurrence rates and local aggressive behavior. To determine whether glucose transporters proteins (GLUT-1 and GLUT-3) and carbonic anhydrase IX (CA IX) are associated with the development of as dentigerous cyst (DC), odontogenic keratocyst (OK), and ameloblastoma (AM), we evaluated the immunohistochemical expression of these proteins in these lesions. MATERIALS AND METHODS: Immunoexpression of GLUT-1, GLUT-3, and CA IX was evaluated semiquantitative fields in each of the 20 cases of OK, AM, and DC. The cases were classified according to the scores: 0 (0% positive cells), 1 (<10% of positive cells), 2 (10-50% of positive cells), and 3 (>50% of positive cells). The statistical analysis was performed using Pearson's chi-square, Kruskal-Wallis and Mann-Whitney tests. RESULTS: All cases were positive for GLUT-1 and 65% of OK showed scored 3. Staining was diffuse in 90% of OK and 85% of DC cases (P < 0.001). In 50% of OK and AM, staining was only observed in the membrane (P = 0.01). Most of the samples (66.7%) were negative for GLUT-3. Staining intensity for anhydrase was higher in the epithelium of DC when compared to OK (P = 0.01). Strong staining was observed in 55% of DC and 20% of OK samples (P = 0.01). CONCLUSIONS: These results suggest that GLUT-1 may be involved in the metabolic regulation of glucose in odontogenic lesions studied. In addition, CA IX appears to influence the development of AM, OK, and DC which can explain the differences their biological behavior.

Assuntos

Ameloblastoma/metabolismo; Anidrase Carbônica IX/metabolismo; Cisto Dentígero/metabolismo; Transportador de Glucose Tipo 1/metabolismo; Transportador de Glucose Tipo 3/metabolismo; Neoplasias Maxilomandibulares/metabolismo; Cistos Odontogênicos/metabolismo; Biomarcadores Tumorais/metabolismo; Humanos; Imuno-Histoquímica

Palavras-chave

carbonic anhydrase IX; glucose transporters; immunohistochemical

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ameloblastoma / Cisto Dentígero / Neoplasias Maxilomandibulares / Cistos Odontogênicos / Transportador de Glucose Tipo 1 / Transportador de Glucose Tipo 3 / Anidrase Carbônica IX Limite: Humans Idioma: En Revista: J Oral Pathol Med Assunto da revista: ODONTOLOGIA / PATOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Dinamarca

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