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Lactobacillus rhamnosus could inhibit Porphyromonas gingivalis derived CXCL8 attenuation.
Mendi, Aysegül; Köse, Sevil; Uçkan, Duygu; Akca, Gülçin; Yilmaz, Dervis; Aral, Levent; Gültekin, Sibel Elif; Eroglu, Tamer; Kiliç, Emine; Uçkan, Sina.
Afiliação
  • Mendi A; Department of Medical Microbiology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
  • Köse S; PEDI-STEM Center for Stem Cell Research and Development, Hacettepe University, Ankara, Turkey.
  • Uçkan D; PEDI-STEM Center for Stem Cell Research and Development, Hacettepe University, Ankara, Turkey.
  • Akca G; Department of Medical Microbiology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
  • Yilmaz D; Department of Oral and Maxilofacial Surgery, Faculty of Dentistry, Gazi University, Ankara, Turkey.
  • Aral L; Department of Oral and Maxilofacial Surgery, Faculty of Dentistry, Gazi University, Ankara, Turkey.
  • Gültekin SE; Department of Oral Pathology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
  • Eroglu T; Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Baskent University, Ankara, Turkey.
  • Kiliç E; PEDI-STEM Center for Stem Cell Research and Development, Hacettepe University, Ankara, Turkey.
  • Uçkan S; Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Baskent University, Ankara, Turkey.
J Appl Oral Sci ; 24(1): 67-75, 2016.
Article em En | MEDLINE | ID: mdl-27008259
UNLABELLED: An increasing body of evidence suggests that the use of probiotic bacteria is a promising intervention approach for the treatment of inflammatory diseases with a polymicrobial etiology. P. gingivalis has been noted to have a different way of interacting with the innate immune response of the host compared to other pathogenic bacteria, which is a recognized feature that inhibits CXCL8 expression. OBJECTIVE: The aim of the study was to determine if P. gingivalis infection modulates the inflammatory response of gingival stromal stem cells (G-MSSCs), including the release of CXCL8, and the expression of TLRs and if immunomodulatory L. rhamnosus ATCC9595 could prevent CXCL8 inhibition in experimental inflammation. MATERIAL AND METHODS: G-MSSCs were pretreated with L. rhamnosus ATCC9595 and then stimulated with P. gingivalis ATCC33277. CXCL8 and IL-10 levels were investigated with ELISA and the TLR-4 and 2 were determined through flow cytometer analysis. RESULTS: CXCL8 was suppressed by P. gingivalis and L. rhamnosus ATCC9595, whereas incubation with both strains did not abolish CXCL8. L. rhamnosus ATCC9595 scaled down the expression of TLR4 and induced TLR2 expression when exposed to P. gingivalis stimulation (p<0.01). CONCLUSIONS: These findings provide evidence that L. rhamnosus ATCC9595 can modulate the inflammatory signals and could introduce P. gingivalis to immune systems by inducing CXCL8 secretion.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interleucina-8 / Porphyromonas gingivalis / Probióticos / Lacticaseibacillus rhamnosus / Células-Tronco Mesenquimais Limite: Adult / Humans Idioma: En Revista: J Appl Oral Sci Assunto da revista: ODONTOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Turquia País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interleucina-8 / Porphyromonas gingivalis / Probióticos / Lacticaseibacillus rhamnosus / Células-Tronco Mesenquimais Limite: Adult / Humans Idioma: En Revista: J Appl Oral Sci Assunto da revista: ODONTOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Turquia País de publicação: Brasil