Your browser doesn't support javascript.
loading
STUDIES OF MOLECULAR CHANGES IN INTERVERTEBRAL DISC DEGENERATION IN ANIMAL MODEL.
de Campos, Marcelo Ferraz; de Oliveira, Cintia Pereira; Neff, Charles Benjamin; Correa, Olga Maria de Toledo; Pinhal, Maria Aparecida Silva; Rodrigues, Luciano Miller Reis.
Afiliação
  • de Campos MF; . Faculdade de Medicina do ABC, Orthopedics Discipline, Santo André, SP, Brazil.
  • de Oliveira CP; . Faculdade de Medicina do ABC, Orthopedics Discipline, Santo André, SP, Brazil.; . Faculdade de Medicina do ABC, Biochemistry Department, Santo André, SP, Brazil.
  • Neff CB; . Faculdade de Medicina do ABC, Orthopedics Discipline, Santo André, SP, Brazil.
  • Correa OM; . Faculdade de Medicina do ABC, Cell Biology Department, Santo André, SP, Brazil.
  • Pinhal MA; . Faculdade de Medicina do ABC, Biochemistry Department, Santo André, SP, Brazil.; . Universidade Federal de São Paulo, Biochemistry Department, São Paulo, SP, Brazil.
  • Rodrigues LM; . Universidade Federal de São Paulo, Biochemistry Department, São Paulo, SP, Brazil.
Acta Ortop Bras ; 24(1): 16-21, 2016.
Article em En | MEDLINE | ID: mdl-26997908
OBJECTIVE: To evaluate the structural and molecular changes in the extracellular matrix (ECM) during the process of intervertebral disc degeneration, using animal model. METHODS: Wistar rats underwent intervertebral disc degeneration through 20-gauge needle puncture, and 360° rotation applied for 30 sec, representing the degenerated group, while control group was not submitted to this procedure. Histological parameters and expression of extracellular matrix molecules were evaluated in the 15(th) and 28(th) days after degenerative induction. RESULTS: Fifteen days after the induction of intervertebral disc degeneration, significant changes were observed, such as reduction in the expression metalloprotease-9 (MMP9) and interleukins (IL-6 and IL-10). There was a significant increase in the expression of vascular endothelial growth factor (VEGF) and caspase-3. However, different alterations in the ECM were observed at 28 days, the level of collagen I, metalloprotease-2 (MMP2) and caspase-3 were enhanced. Furthermore, expression of heparanase isoforms (HPSE1 and HPSE2) mRNA were increased in the degenerative intervertebral disc. CONCLUSION: The different profiles of ECM molecules observed during the intervertebral disc degeneration suggest that molecular processes such as ECM remodeling, neovascularization, apoptosis and inflammation occur. Experimental Study.
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Acta Ortop Bras Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Acta Ortop Bras Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil