Synergistic activity for high-proliferative-potential colony-forming cell (HPP-CFC) development, stable to trypsin digestion, present in pregnant mouse uterus and placenta extract.
Acta Physiol Pharmacol Latinoam
; 39(3): 315-23, 1989.
Article
em En
| MEDLINE
| ID: mdl-2699385
In this paper we describe a crude pregnant mouse uterus and embryo extract (PMUE) prepared from CFW/ep mice which was able to stimulate the proliferation of high-proliferative-potential colony-forming cells (HPP-CFC) of bone marrow of normal mice, in vitro, in semisolid agar culture system. The development of that primitive murine progenitor cells requires the presence of a macrophage-stimulating factor (CSF-1) plus a synergistic factor (SF). The biological activity of both factors was present in our extracts. The higher SF activity was found in uterine plus placental tissues extracts. The SF was precipitated over 45 per cent ammonium sulfate saturation, and behaved as a nondialyzable substance, remained unaffected by trypsin digestion, and was heat-stable (70 degrees C for 15 min).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Útero
/
Células da Medula Óssea
/
Fatores Estimuladores de Colônias
/
Embrião de Mamíferos
Limite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Acta Physiol Pharmacol Latinoam
Assunto da revista:
FARMACOLOGIA
/
FISIOLOGIA
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Argentina