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Enzymatic hydrolysis of chitin pretreated by rapid depressurization from supercritical 1,1,1,2-tetrafluoroethane toward highly acetylated oligosaccharides.
Villa-Lerma, Guadalupe; González-Márquez, Humberto; Gimeno, Miquel; Trombotto, Stéphane; David, Laurent; Ifuku, Shinsuke; Shirai, Keiko.
Afiliação
  • Villa-Lerma G; Universidad Autónoma Metropolitana, Biotechnology Department, Laboratory of Biopolymers and Pilot Plant of Bioprocessing of Agro-Industrial and Food By-Products, Av. San Rafael Atlixco No. 186, Col. Vicentina, C.P. 09340, Iztapalapa, México City, Mexico.
  • González-Márquez H; Universidad Autónoma Metropolitana, Biotechnology Department, Laboratory of Biopolymers and Pilot Plant of Bioprocessing of Agro-Industrial and Food By-Products, Av. San Rafael Atlixco No. 186, Col. Vicentina, C.P. 09340, Iztapalapa, México City, Mexico.
  • Gimeno M; Universidad Nacional Autónoma de México, Facultad de Química, Ciudad Universitaria, C.P. 04510, Mexico City, Mexico.
  • Trombotto S; Ingénierie des Matériaux Polymères IMP@Lyon1, UMR CNRS 5223, Université Claude Bernard Lyon 1, Université de Lyon, 15 bd A. Latarjet, Villeurbanne Cedex, France.
  • David L; Ingénierie des Matériaux Polymères IMP@Lyon1, UMR CNRS 5223, Université Claude Bernard Lyon 1, Université de Lyon, 15 bd A. Latarjet, Villeurbanne Cedex, France.
  • Ifuku S; Graduate School of Engineering, Department of Chemistry and Biotechnology, Tottori University, Koyamacho-minami 4-101, Tottori City, Tottori Prefecture 680-8550, Japan.
  • Shirai K; Universidad Autónoma Metropolitana, Biotechnology Department, Laboratory of Biopolymers and Pilot Plant of Bioprocessing of Agro-Industrial and Food By-Products, Av. San Rafael Atlixco No. 186, Col. Vicentina, C.P. 09340, Iztapalapa, México City, Mexico. Electronic address: smk@xanum.uam.mx.
Bioresour Technol ; 209: 180-6, 2016 Jun.
Article em En | MEDLINE | ID: mdl-26970920
The hydrolysis of chitin treated under supercritical conditions was successfully carried out using chitinases obtained by an optimized fermentation of the fungus Lecanicillium lecanii. The biopolymer was subjected to a pretreatment based on suspension in supercritical 1,1,1,2-tetrafluoroethane (scR134a), which possesses a critical temperature and pressure of 101°C and 40bar, respectively, followed by rapid depressurization to atmospheric pressure and further fibrillation. This methodology was compared to control untreated chitins and chitin subjected to steam explosion showing improved production of reducing sugars (0.18mg/mL), enzymatic hydrolysis and high acetylation (FA of 0.45) in products with degrees of polymerization between 2 and 5.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Quitina / Quitinases / Hidrocarbonetos Fluorados Idioma: En Revista: Bioresour Technol Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Quitina / Quitinases / Hidrocarbonetos Fluorados Idioma: En Revista: Bioresour Technol Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido