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Reproductive alterations in hyperinsulinemic but normoandrogenic MSG obese female rats.
Gaspar, Renato Simões; Benevides, Renata Ohana Alves; Fontelles, João Lucas de Lima; Vale, Caroline Castro; França, Lucas Martins; Barros, Paulo de Tarso Silva; Paes, Antonio Marcus de Andrade.
Afiliação
  • Gaspar RS; Department of Physiological SciencesFederal University of Maranhão, São Luís, Maranhão, Brazil.
  • Benevides RO; Department of Physiological SciencesFederal University of Maranhão, São Luís, Maranhão, Brazil.
  • Fontelles JL; Department of Physiological SciencesFederal University of Maranhão, São Luís, Maranhão, Brazil.
  • Vale CC; Department of Physiological SciencesFederal University of Maranhão, São Luís, Maranhão, Brazil.
  • França LM; Department of Physiological SciencesFederal University of Maranhão, São Luís, Maranhão, Brazil.
  • Barros Pde T; Department of Physiological SciencesFederal University of Maranhão, São Luís, Maranhão, Brazil Department of PhysiologySchool of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
  • Paes AM; Department of Physiological SciencesFederal University of Maranhão, São Luís, Maranhão, Brazil marcuspaes@ufma.br.
J Endocrinol ; 229(2): 61-72, 2016 05.
Article em En | MEDLINE | ID: mdl-26952035
Obesity and metabolic syndrome are the common causes of reproductive and fertility disorders in women. In particular, polycystic ovary syndrome, which is clinically characterized by hyperandrogenism, oligo/anovulation, and polycystic ovarian morphology, has been increasingly associated with metabolic disorders. However, given the broad interplay between metabolic and reproductive functions, this remains a field of intense research. In this study, we investigated the effect of monosodium l-glutamate (MSG)-induced obesity on reproductive biology of female rats. Newborn female rats were subcutaneously injected with MSG (4g/kg/day) or equiosmolar saline (CTR) each 2 days up to postnatal day (pnd) 10. On pnd 60, estrous cycle was evaluated using vaginal smears twice a day for 15 days, which showed MSG rats to be oligocyclic. Thereafter, animals were killed on estrous phase for blood and tissue collection. MSG rats had increased body mass, accumulation of retroperitoneal and visceral fat pads, and visceral adipocyte hypertrophy compared with CTR rats. MSG rats were also dyslipidemic and hyperinsulinemic but were normoglycemic and normoandrogenic. Ovarian morphology analysis showed that MSG rats had a two-fold decrease in oocyte count but a six-fold increase on ovarian follicular cysts, along with a higher number of total primordial and atretic follicles. Moreover, MSG rats had a four-fold increase in anti-Müllerian hormone immunohistochemical staining on antral follicles. Taken together, data presented here characterize MSG obesity as a unique model to study the metabolic pathways underlying reproductive disorders in the absence of overactivated hypothalamic-pituitary-gonadal axis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glutamato de Sódio / Obesidade Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Endocrinol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glutamato de Sódio / Obesidade Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Endocrinol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido