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Identification of causative Leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP.
Koarashi, Yu; Cáceres, Abraham G; Saca, Florencia Margarita Zúniga; Flores, Elsa Elvira Palacios; Trujillo, Adela Celis; Alvares, José Luis Abanto; Yoshimatsu, Kumiko; Arikawa, Jiro; Katakura, Ken; Hashiguchi, Yoshihisa; Kato, Hirotomo.
Afiliação
  • Koarashi Y; Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan; Department of Microbiology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
  • Cáceres AG; Sección de Entomología, Instituto de Medicina Tropical "Daniel A. Carrión" y Departamento Académico de Microbiología Médica, Facultad de Medicina Humana, Universidad Nacional Mayor de San Marcos, Lima, Peru; Laboratorio de Entomología, Instituto Nacional de Salud, Lima, Peru.
  • Saca FMZ; Laboratorio de Referencia Regional de Huánuco, DIRESA Huánuco, Peru.
  • Flores EEP; Dirección Ejecutiva de Epidemiologia, DIRESA Huánuco, Peru.
  • Trujillo AC; Dirección Ejecutiva de Epidemiologia, DIRESA Huánuco, Peru.
  • Alvares JLA; Vigilancia Entomológica y Control Vectorial, Dirección de Saneamiento Ambiental, DIRESA Huánuco, Peru.
  • Yoshimatsu K; Department of Microbiology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
  • Arikawa J; Department of Microbiology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
  • Katakura K; Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
  • Hashiguchi Y; Parasitologia y Medicina Tropical, Facultad de Medicina, Universidad Catolica de Santiago de Guayaquil, Guayaquil, Ecuador; Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan.
  • Kato H; Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan. Electronic address: hkato@vetmed.hokudai.ac.jp.
Acta Trop ; 158: 83-87, 2016 Jun.
Article em En | MEDLINE | ID: mdl-26943992
A PCR-Restriction Fragment Length Polymorphism (RFLP) targeting the mannose phosphate isomerase gene was established to differentiate Leishmania species distributed near the Department of Huanuco, Peru. The technique was applied to 267 DNA samples extracted from Giemsa-stained smears of cutaneous lesions taken from patients suspected for cutaneous leishmaniasis in the area, and the present status of causative Leishmania species was identified. Of 114 PCR-amplified samples, 22, 19, 24 and 49 samples were identified to be infected by Leishmania (Viannia) braziliensis, L. (V.) peruviana, L. (V.) guyanensis, and a hybrid of L. (V.) braziliensis/L. (V.) peruviana, respectively, and the validity of PCR-RFLP was confirmed by sequence analysis. Since PCR-RFLP is simple and rapid, the technique will be a useful tool for the epidemiological study of leishmaniasis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leishmaniose Cutânea / Leishmania Tipo de estudo: Diagnostic_studies Limite: Humans País/Região como assunto: America do sul / Peru Idioma: En Revista: Acta Trop Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Japão País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leishmaniose Cutânea / Leishmania Tipo de estudo: Diagnostic_studies Limite: Humans País/Região como assunto: America do sul / Peru Idioma: En Revista: Acta Trop Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Japão País de publicação: Holanda