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In Vitro Oxidation of Collagen Promotes the Formation of Advanced Oxidation Protein Products and the Activation of Human Neutrophils.
Bochi, Guilherme Vargas; Torbitz, Vanessa Dorneles; de Campos, Luízi Prestes; Sangoi, Manuela Borges; Fernandes, Natieli Flores; Gomes, Patrícia; Moretto, Maria Beatriz; Barbisan, Fernanda; da Cruz, Ivana Beatrice Mânica; Moresco, Rafael Noal.
Afiliação
  • Bochi GV; Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
  • Torbitz VD; Pharmacology Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
  • de Campos LP; Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
  • Sangoi MB; Pharmaceutical Sciences Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
  • Fernandes NF; Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
  • Gomes P; Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
  • Moretto MB; Pharmaceutical Sciences Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
  • Barbisan F; Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
  • da Cruz IB; Nanosciences Postgraduate Program, Franciscan University Center, Santa Maria, Brazil.
  • Moresco RN; Pharmacology Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
Inflammation ; 39(2): 916-27, 2016 Apr.
Article em En | MEDLINE | ID: mdl-26920846
The accumulation of advanced oxidation protein products (AOPPs) has been linked to several pathological conditions. Here, we investigated collagen as a potential source for AOPP formation and determined the effects of hypochlorous acid (HOCl)-treated collagen (collagen-AOPPs) on human neutrophil activity. We also assessed whether alpha-tocopherol could counteract these effects. Exposure to HOCl increased the levels of collagen-AOPPs. Collagen-AOPPs also stimulated the production of AOPPs, nitric oxide (NO), superoxide radicals (O2(-)), and HOCl by neutrophils. Collagen-AOPPs induced apoptosis and decreased the number of viable cells. Alpha-tocopherol prevented the formation of collagen-AOPPs, strongly inhibited the collagen-AOPP-induced production of O2(-) and HOCl, and increased the viability of neutrophils. Our results suggest that collagen is an important protein that interacts with HOCl to form AOPPs, and consequently, collagen-AOPP formation is related to human neutrophil activation and cell death.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Colágeno / Ácido Hipocloroso / Ativação de Neutrófilo / Alfa-Tocoferol / Produtos da Oxidação Avançada de Proteínas / Neutrófilos Limite: Humans Idioma: En Revista: Inflammation Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Colágeno / Ácido Hipocloroso / Ativação de Neutrófilo / Alfa-Tocoferol / Produtos da Oxidação Avançada de Proteínas / Neutrófilos Limite: Humans Idioma: En Revista: Inflammation Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos