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Method Based on the ß-Lactamase PenPC Fluorescent Labeled for ß-Lactam Antibiotic Quantification in Human Plasma.
Andresen, Max; Wong, Kwok-Yin; Leung, Yun-Chung; Wong, Wai-Ting; Chan, Pak-Ho; Andresen-Vasquez, Max; Alegria, Leyla; Silva, Camila; Tapia, Pablo; Downey, Patricio; Soto, Dagoberto.
Afiliação
  • Andresen M; Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • Wong KY; State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
  • Leung YC; State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
  • Wong WT; State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
  • Chan PH; State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
  • Andresen-Vasquez M; Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • Alegria L; Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • Silva C; Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • Tapia P; Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • Downey P; Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • Soto D; Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
Biomed Res Int ; 2016: 4307987, 2016.
Article em En | MEDLINE | ID: mdl-26904674
Recently, Wong et al. have successfully developed a fluorescent biosensor based on the PenPC ß-lactamase which changes its intrinsic fluorescence in presence of ß-lactam antibiotics (BLAs). Here, we studied systematically this correlation among the fluorescence change of the biosensor and the concentration of different BLAs aimed at developing a novel method for estimating the concentration of a wide range of BLAs. This method showed high precision and specificity and very low interference from clinically relevant samples. We were able to monitor the pharmacokinetics of meropenem in healthy volunteers as well as in an ill animal model too, indicating that the implemented method could be suitable for clinical practice.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Tienamicinas / Beta-Lactamas / Antibacterianos Limite: Animals / Humans Idioma: En Revista: Biomed Res Int Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Tienamicinas / Beta-Lactamas / Antibacterianos Limite: Animals / Humans Idioma: En Revista: Biomed Res Int Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos