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ß2-Adrenoceptor is involved in connective tissue remodeling in regenerating muscles by decreasing the activity of MMP-9.
Silva, Meiricris T; Nascimento, Tábata L; Pereira, Marcelo G; Siqueira, Adriane S; Brum, Patrícia C; Jaeger, Ruy G; Miyabara, Elen H.
Afiliação
  • Silva MT; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 2415, 05508-000, São Paulo, SP, Brazil.
  • Nascimento TL; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 2415, 05508-000, São Paulo, SP, Brazil.
  • Pereira MG; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 2415, 05508-000, São Paulo, SP, Brazil.
  • Siqueira AS; Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Brum PC; Department of Biodynamics, School of Physical Education and Sport, University of São Paulo, São Paulo, Brazil.
  • Jaeger RG; Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  • Miyabara EH; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 2415, 05508-000, São Paulo, SP, Brazil. elenm@usp.br.
Cell Tissue Res ; 365(1): 173-86, 2016 07.
Article em En | MEDLINE | ID: mdl-26896238
We investigated the role of ß2-adrenoceptors in the connective tissue remodeling of regenerating muscles from ß2-adrenoceptor knockout (ß2KO) mice. Tibialis anterior muscles from ß2KO mice were cryolesioned and analyzed after 3, 10, and 21 days. Regenerating muscles from ß2KO mice showed a significant increase in the area density of the connective tissue and in the amount of collagen at 10 days compared with wild-type (WT) mice. A greater increase occurred in the expression levels of collagen I, III, and IV in regenerating muscles from ß2KO mice evaluated at 10 days compared with WT mice; this increase continued at 21 days, except for collagen III. Matrix metalloproteinase (MMP-2) activity increased to a similar extent in regenerating muscles from both ß2KO and WT mice at 3 and 10 days. This was also the case for MMP-9 activity in regenerating muscles from both ß2KO and WT mice at 3 days; however, at 10 days post-cryolesion, this activity returned to baseline levels only in WT mice. MMP-3 activity was unaltered in regenerating muscles at 10 days. mRNA levels of tumor necrosis factor-α increased in regenerating muscles from WT and ß2KO mice at 3 days and, at 10 days post-cryolesion, returned to baseline only in WT mice. mRNA levels of interleukin-6 increased in muscles from WT mice at 3 days post-cryolesion and returned to baseline at 10 days post-cryolesion but were unchanged in ß2KO mice. Our results suggest that the ß2-adrenoceptor contributes to collagen remodeling during muscle regeneration by decreasing MMP-9 activity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regeneração / Receptores Adrenérgicos beta 2 / Músculo Esquelético / Tecido Conjuntivo / Metaloproteinase 9 da Matriz Limite: Animals Idioma: En Revista: Cell Tissue Res Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regeneração / Receptores Adrenérgicos beta 2 / Músculo Esquelético / Tecido Conjuntivo / Metaloproteinase 9 da Matriz Limite: Animals Idioma: En Revista: Cell Tissue Res Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha