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Merida virus, a putative novel rhabdovirus discovered in Culex and Ochlerotatus spp. mosquitoes in the Yucatan Peninsula of Mexico.
Charles, Jermilia; Firth, Andrew E; Loroño-Pino, Maria A; Garcia-Rejon, Julian E; Farfan-Ale, Jose A; Lipkin, W Ian; Blitvich, Bradley J; Briese, Thomas.
Afiliação
  • Charles J; Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
  • Firth AE; Department of Pathology, University of Cambridge, Cambridge, UK.
  • Loroño-Pino MA; Laboratorio de Arbovirología, Centro de Investigaciones Regionales Dr Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
  • Garcia-Rejon JE; Laboratorio de Arbovirología, Centro de Investigaciones Regionales Dr Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
  • Farfan-Ale JA; Laboratorio de Arbovirología, Centro de Investigaciones Regionales Dr Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
  • Lipkin WI; Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
  • Blitvich BJ; Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
  • Briese T; Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
J Gen Virol ; 97(4): 977-987, 2016 Apr.
Article em En | MEDLINE | ID: mdl-26868915
Sequences corresponding to a putative, novel rhabdovirus [designated Merida virus (MERDV)] were initially detected in a pool of Culex quinquefasciatus collected in the Yucatan Peninsula of Mexico. The entire genome was sequenced, revealing 11 798 nt and five major ORFs, which encode the nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G) and RNA-dependent RNA polymerase (L). The deduced amino acid sequences of the N, G and L proteins have no more than 24, 38 and 43 % identity, respectively, to the corresponding sequences of all other known rhabdoviruses, whereas those of the P and M proteins have no significant identity with any sequences in GenBank and their identity is only suggested based on their genome position. Using specific reverse transcription-PCR assays established from the genome sequence, 27 571 C. quinquefasciatus which had been sorted in 728 pools were screened to assess the prevalence of MERDV in nature and 25 pools were found positive. The minimal infection rate (calculated as the number of positive mosquito pools per 1000 mosquitoes tested) was 0.9, and similar for both females and males. Screening another 140 pools of 5484 mosquitoes belonging to four other genera identified positive pools of Ochlerotatus spp. mosquitoes, indicating that the host range is not restricted to C. quinquefasciatus. Attempts to isolate MERDV in C6/36 and Vero cells were unsuccessful. In summary, we provide evidence that a previously undescribed rhabdovirus occurs in mosquitoes in Mexico.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Rhabdoviridae / Proteínas Virais / RNA Viral / Genoma Viral / Insetos Vetores Tipo de estudo: Risk_factors_studies Limite: Animals País/Região como assunto: Mexico Idioma: En Revista: J Gen Virol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Rhabdoviridae / Proteínas Virais / RNA Viral / Genoma Viral / Insetos Vetores Tipo de estudo: Risk_factors_studies Limite: Animals País/Região como assunto: Mexico Idioma: En Revista: J Gen Virol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido