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A molecular platform for the diagnosis of multidrug-resistant and pre-extensively drug-resistant tuberculosis based on single nucleotide polymorphism mutations present in Colombian isolates of Mycobacterium tuberculosis.
Martínez, Luz Maira Wintaco; Castro, Gloria Puerto; Guerrero, Martha Inírida.
Afiliação
  • Martínez LM; Instituto Nacional de Salud, Bogotá, Colombia.
  • Castro GP; Instituto Nacional de Salud, Bogotá, Colombia.
  • Guerrero MI; Instituto Nacional de Salud, Bogotá, Colombia.
Mem Inst Oswaldo Cruz ; 111(2): 93-100, 2016 Feb.
Article em En | MEDLINE | ID: mdl-26841047
Developing a fast, inexpensive, and specific test that reflects the mutations present in Mycobacterium tuberculosis isolates according to geographic region is the main challenge for drug-resistant tuberculosis (TB) control. The objective of this study was to develop a molecular platform to make a rapid diagnosis of multidrug-resistant (MDR) and extensively drug-resistant TB based on single nucleotide polymorphism (SNP) mutations present in therpoB, katG, inhA,ahpC, and gyrA genes from Colombian M. tuberculosis isolates. The amplification and sequencing of each target gene was performed. Capture oligonucleotides, which were tested before being used with isolates to assess the performance, were designed for wild type and mutated codons, and the platform was standardised based on the reverse hybridisation principle. This method was tested on DNA samples extracted from clinical isolates from 160 Colombian patients who were previously phenotypically and genotypically characterised as having susceptible or MDR M. tuberculosis. For our method, the kappa index of the sequencing results was 0,966, 0,825, 0,766, 0,740, and 0,625 forrpoB, katG, inhA,ahpC, and gyrA, respectively. Sensitivity and specificity were ranked between 90-100% compared with those of phenotypic drug susceptibility testing. Our assay helps to pave the way for implementation locally and for specifically adapted methods that can simultaneously detect drug resistance mutations to first and second-line drugs within a few hours.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Bacteriano / Tuberculose Resistente a Múltiplos Medicamentos / Polimorfismo de Nucleotídeo Único / Técnicas de Diagnóstico Molecular / Mutação / Mycobacterium tuberculosis Tipo de estudo: Diagnostic_studies Limite: Humans País/Região como assunto: America do sul / Colombia Idioma: En Revista: Mem Inst Oswaldo Cruz Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Bacteriano / Tuberculose Resistente a Múltiplos Medicamentos / Polimorfismo de Nucleotídeo Único / Técnicas de Diagnóstico Molecular / Mutação / Mycobacterium tuberculosis Tipo de estudo: Diagnostic_studies Limite: Humans País/Região como assunto: America do sul / Colombia Idioma: En Revista: Mem Inst Oswaldo Cruz Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Brasil