Your browser doesn't support javascript.
loading
Norovirus molecular detection in Uruguayan sewage samples reveals a high genetic diversity and GII.4 variant replacement along time.
Victoria, M; Tort, L F L; Lizasoain, A; García, M; Castells, M; Berois, M; Divizia, M; Leite, J P G; Miagostovich, M P; Cristina, J; Colina, R.
Afiliação
  • Victoria M; Laboratory of Molecular Virology, Department of Biological Sciences, Regional Norte, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay.
  • Tort LF; Laboratory of Molecular Virology, Department of Biological Sciences, Regional Norte, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay.
  • Lizasoain A; Laboratory of Molecular Virology, Department of Biological Sciences, Regional Norte, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay.
  • García M; Laboratory of Molecular Virology, Department of Biological Sciences, Regional Norte, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay.
  • Castells M; Laboratory of Molecular Virology, Department of Biological Sciences, Regional Norte, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay.
  • Berois M; Virology Section, School of Sciences, Universidad de la República, Montevideo, Uruguay.
  • Divizia M; Laboratory of Environmental Virology, Department of Experimental Medicine and Surgery, Tor Vergata University, Rome, Italy.
  • Leite JP; Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
  • Miagostovich MP; Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
  • Cristina J; Laboratory of Molecular Virology, Nuclear Investigation Center, School of Sciences, Universidad de la República, Montevideo, Uruguay.
  • Colina R; Laboratory of Molecular Virology, Department of Biological Sciences, Regional Norte, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay.
J Appl Microbiol ; 120(5): 1427-35, 2016 May.
Article em En | MEDLINE | ID: mdl-26784369
AIMS: To determine the prevalence and molecular epidemiology of norovirus (NoV) genogroup I (GI) and GII in Uruguay. METHODS AND RESULTS: One hundred and sixteen sewage samples were collected in six cities (Bella Unión, Salto, Paysandú, Fray Bentos, Melo and Treinta y Tres) from March 2011 to April 2013, viruses were concentrated by ultracentrifugation and NoV studies were performed by semi-nested RT-PCR (partial capsid region). NoV were detected in samples from all the cities and detected in 72% (84/116) of the samples with nine of them belonging to GI, 48 to GII and 27 to both genogroups. Remarkably, a high genetic diversity was identified: GII.2 (n = 13), GII.4 (n = 13), GI.1 (n = 5), GI.4 (n = 5), GI.8 (n = 4), GII.13 (n = 4), GII.1 (n = 3), GII.6 (n = 3), GI.3 (n = 1), GI.5 (n = 1), GI.6 (n = 1), GII.3 (n = 1), GII.17 (n = 1). Interestingly, a complete replacement of GII.4 New Orleans 2009 by GII.4 Sydney 2012 variants during 2012 was evidenced. CONCLUSION: This study reveals a high circulation of different NoV GI and GII genotypes in sewage evidencing a replacement of GII.4 variants. SIGNIFICANCE AND IMPACT OF STUDY: This approach can be used as an indicator of the presence of a new GII.4 variant which can originate an increase in acute gastroenteritis outbreaks worldwide.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Esgotos / Variação Genética / Norovirus Tipo de estudo: Diagnostic_studies / Risk_factors_studies País/Região como assunto: America do sul / Uruguay Idioma: En Revista: J Appl Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Uruguai País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Esgotos / Variação Genética / Norovirus Tipo de estudo: Diagnostic_studies / Risk_factors_studies País/Região como assunto: America do sul / Uruguay Idioma: En Revista: J Appl Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Uruguai País de publicação: Reino Unido