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Detection of a mutation at codon 43 of the rpsL gene in Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae by PCR-RFLP.
Zhang, Y; Yang, X; Zhou, F Y; Zhang, A F; Zhu, X F; Chen, Y; Zhou, M G; Gao, T C.
Afiliação
  • Zhang Y; Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei, China.
  • Yang X; College of Crop Protection, Nanjing Agricultural University, Nanjing, China.
  • Zhou FY; Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei, China.
  • Zhang AF; Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei, China.
  • Zhu XF; Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei, China.
  • Chen Y; College of Crop Protection, Nanjing Agricultural University, Nanjing, China.
  • Zhou MG; Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei, China.
  • Gao TC; College of Crop Protection, Nanjing Agricultural University, Nanjing, China.
Genet Mol Res ; 14(4): 18587-95, 2015 Dec 29.
Article em En | MEDLINE | ID: mdl-26782507
The aim of this study was to develop a method to detect a point mutation in the ribosomal S12 protein (rpsL) gene in streptomycin-resistant strains of Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to detect a point mutation in codon 43 of the rpsL gene in X. oryzae pv. oryzicola and X. oryzae pv. oryzae. The 304-bp PCR product from the rpsL gene was digested by MboII to form two fragments (201 and 103 bp) if there was a mutation at codon 43, or three fragments (146, 103, and 55 bp) if there was no mutation. Compared with the results from nucleotide sequencing, the PCR-RFLP method was accurate in detecting the point mutation at codon 43 of the rpsL gene in streptomycin-resistant strains of X. oryzae pv. oryzicola and X. oryzae pv. oryzae. These results indicate that the PCR-RFLP is a simple, rapid and reliable method for detecting the point mutation at codon 43 of the rpsL gene.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Ribossômicas / Proteínas de Bactérias / Xanthomonas / Códon / Mutação Tipo de estudo: Diagnostic_studies Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Ribossômicas / Proteínas de Bactérias / Xanthomonas / Códon / Mutação Tipo de estudo: Diagnostic_studies Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil