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Protective efficacy of a genetic subunit bacterin against edema disease of swine in mice.
Yang, X L; Liu, Y J; Hu, L Q; Guo, L W; Liu, G P; Wu, B; Chen, H C.
Afiliação
  • Yang XL; College of Animal Science, Yangtze University, JingZhou, HuBei, China.
  • Liu YJ; Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
  • Hu LQ; Center for Animal Disease Control and Prevention of China, Beijing, China.
  • Guo LW; College of Animal Science, Yangtze University, JingZhou, HuBei, China.
  • Liu GP; Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
  • Wu B; College of Animal Science, Yangtze University, JingZhou, HuBei, China.
  • Chen HC; Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
Genet Mol Res ; 14(4): 18026-33, 2015 Dec 22.
Article em En | MEDLINE | ID: mdl-26782450
The exotoxin SLT-IIeB from the Escherichia coli Ee strain was expressed in E. coli, and the recombinant protein was purified, mixed with the Ee strain, then emulsified with oil-emulsion adjuvants to obtain a mixed subunit bacterin. Groups of Kunming mice were immunized at weeks 0 and 2, and challenged intraperitoneally with the Ee strain at week 4. Antibodies were detected by ELISA and an agglutination test. After the second immunization, the antibody level increased and the rate of immune protection against the Ee strain was 70 and 91.7% in the subunit bacterin and bacterin groups, respectively. Therefore, the mixed subunit bacterin provided good protection against the homologous Ee strain, which provides a basis for further research, into high-efficacy vaccines against porcine edema disease.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vacinas Bacterianas / Toxina Shiga II / Edematose Suína / Infecções por Escherichia coli Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vacinas Bacterianas / Toxina Shiga II / Edematose Suína / Infecções por Escherichia coli Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil