Comparative effects of vitamin C on the effects of local anesthetics ropivacaine, bupivacaine, and lidocaine on human chondrocytes.
Braz J Anesthesiol
; 66(1): 29-36, 2016.
Article
em En
| MEDLINE
| ID: mdl-26768927
BACKGROUND: Intra-articular injections of local anesthetics are commonly used to enhance post-operative analgesia following orthopedic surgery as arthroscopic surgeries. Nevertheless, recent reports of severe complications due to the use of intra-articular local anesthetic have raised concerns. OBJECTIVES: The study aims to assess use of vitamin C in reducing adverse effects of the most commonly employed anesthetics - ropivacaine, bupivacaine and lidocaine - on human chondrocytes. METHODS: The chondrocyte viability following exposure to 0.5% bupivacaine or 0.75% ropivacaine or 1.0% lidocaine and/or vitamin C at doses 125, 250 and 500 µM was determined by LIVE/DEAD assay and annexin V staining. Expression levels of caspases 3 and 9 were assessed using antibodies by Western blotting. Flow cytometry was performed to analyze the generation of reactive oxygen species. RESULTS: On exposure to the local anesthetics, chondrotoxicity was found in the order ropivacaine
AssuntosPalavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Ascórbico
/
Espécies Reativas de Oxigênio
/
Condrócitos
/
Anestésicos Locais
Limite:
Humans
Idioma:
En
Revista:
Braz J Anesthesiol
Ano de publicação:
2016
Tipo de documento:
Article
País de publicação:
Brasil
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Ascórbico
/
Espécies Reativas de Oxigênio
/
Condrócitos
/
Anestésicos Locais
Limite:
Humans
Idioma:
En
Revista:
Braz J Anesthesiol
Ano de publicação:
2016
Tipo de documento:
Article
País de publicação:
Brasil