[Use of an inhibition method of ELISA in the serologic diagnosis of dengue. Preliminary report]. / Utilización de un método de inhibición de ELISA en el diagnóstico serológico del dengue. Reporte preliminar.
Rev Cubana Med Trop
; 41(1): 18-26, 1989.
Article
em Es
| MEDLINE
| ID: mdl-2672168
This paper reports the standardization of an ELISA (enzyme-linked immunosorbent assay) inhibition method for the serologic diagnosis of dengue, with the use of non-purified antigen. The conditions and criteria of the test for detection of antibodies for a group of 82 sera (12 monosera and 35 paired sera) are established. The results are compared with those obtained by hemagglutination inhibition and a good agreement between the two tests, as well as greater sensitivity of ELISA, is found. Some advantages of this technique in connection with hemagglutination inhibition are listed; they are: easy performance, rapidity, no need for serum treatment, and the use of viral suspensions without using any method of extraction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Testes de Inibição da Hemaglutinação
/
Dengue
/
Vírus da Dengue
/
Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
Es
Revista:
Rev Cubana Med Trop
Ano de publicação:
1989
Tipo de documento:
Article
País de publicação:
Cuba