Trypanosoma cruzi: involvement of proteolytic activity during cell fusion induced by epimastigote form.
Mol Cell Biochem
; 86(2): 189-200, 1989 Apr 11.
Article
em En
| MEDLINE
| ID: mdl-2671665
Human erythrocytes were fused by Trypanosoma cruzi from 7 and 14 day old culture (stationary and declination phases, respectively) while only lysis was induced by 4 day old culture parasite (exponential phase). Lysis and erythrocyte fusion were studied by phase contrast microscopy, measuring of hemolysis and gel electrophoresis. The fusogenicity is Ca2+-dependent while lysis is delayed in the absence of exogenous Ca2+. The proteolysis of erythrocyte protein bands 1, 2, 2.1, 2.3 and 3 are common features of both fusion and lysis processes. Nevertheless the breakdown rate of ankyrin (band 2.1) and band 3 are different in fused or in lysed cells. The lysis process is associated with a faster degradation of band 2.1 and increase of band 2.3 than in the case of the fusion process. By contrast, degradation of band 3 occurs faster in the fusion than in the lytic event. Treatment of fusogenic parasites but not erythrocytes with TPCK, soybean trypsin inhibitor or FCS inhibited to some extent the fusion process and the decrease of bands 1, 2, 2.1, 2.3 and 3. The results suggest that proteases from fusogenic parasites may be directly or indirectly involved in the proteolysis of band 2.1 in a way related to induction of fusion.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endopeptidases
/
Trypanosoma cruzi
/
Membrana Eritrocítica
/
Proteínas de Membrana
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Mol Cell Biochem
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Holanda