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New Coffee Plant-Infecting Xylella fastidiosa Variants Derived via Homologous Recombination.
Jacques, Marie-Agnès; Denancé, Nicolas; Legendre, Bruno; Morel, Emmanuelle; Briand, Martial; Mississipi, Stelly; Durand, Karine; Olivier, Valérie; Portier, Perrine; Poliakoff, Françoise; Crouzillat, Dominique.
Afiliação
  • Jacques MA; INRA, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Beaucouzé, France marie-agnes.jacques@angers.inra.fr.
  • Denancé N; INRA, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Beaucouzé, France Anses Laboratoire de la Santé des Végétaux, Angers, France.
  • Legendre B; Anses Laboratoire de la Santé des Végétaux, Angers, France.
  • Morel E; Nestlé R&D Tours, Tours, France.
  • Briand M; INRA, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Beaucouzé, France.
  • Mississipi S; INRA, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Beaucouzé, France Anses Laboratoire de la Santé des Végétaux, Angers, France Nestlé R&D Tours, Tours, France.
  • Durand K; INRA, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Beaucouzé, France.
  • Olivier V; Anses Laboratoire de la Santé des Végétaux, Angers, France.
  • Portier P; INRA, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Beaucouzé, France.
  • Poliakoff F; Anses Laboratoire de la Santé des Végétaux, Angers, France.
  • Crouzillat D; Nestlé R&D Tours, Tours, France.
Appl Environ Microbiol ; 82(5): 1556-68, 2015 Dec 28.
Article em En | MEDLINE | ID: mdl-26712553
Xylella fastidiosa is a xylem-limited phytopathogenic bacterium endemic to the Americas that has recently emerged in Asia and Europe. Although this bacterium is classified as a quarantine organism in the European Union, importation of plant material from contaminated areas and latent infection in asymptomatic plants have engendered its inevitable introduction. In 2012, four coffee plants (Coffea arabica and Coffea canephora) with leaf scorch symptoms growing in a confined greenhouse were detected and intercepted in France. After identification of the causal agent, this outbreak was eradicated. Three X. fastidiosa strains were isolated from these plants, confirming a preliminary identification based on immunology. The strains were characterized by multiplex PCR and by multilocus sequence analysis/typing (MLSA-MLST) based on seven housekeeping genes. One strain, CFBP 8073, isolated from C. canephora imported from Mexico, was assigned to X. fastidiosa subsp. fastidiosa/X. fastidiosa subsp. sandyi. This strain harbors a novel sequence type (ST) with novel alleles at two loci. The two other strains, CFBP 8072 and CFBP 8074, isolated from Coffea arabica imported from Ecuador, were allocated to X. fastidiosa subsp. pauca. These two strains shared a novel ST with novel alleles at two loci. These MLST profiles showed evidence of recombination events. We provide genome sequences for CFBP 8072 and CFBP 8073 strains. Comparative genomic analyses of these two genome sequences with publicly available X. fastidiosa genomes, including the Italian strain CoDiRO, confirmed these phylogenetic positions and provided candidate alleles for coffee plant adaptation. This study demonstrates the global diversity of X. fastidiosa and highlights the diversity of strains isolated from coffee plants.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Variação Genética / Café / Xylella / Recombinação Homóloga Tipo de estudo: Prognostic_studies País/Região como assunto: America do sul / Ecuador / Europa / Mexico Idioma: En Revista: Appl Environ Microbiol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: França País de publicação: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Variação Genética / Café / Xylella / Recombinação Homóloga Tipo de estudo: Prognostic_studies País/Região como assunto: America do sul / Ecuador / Europa / Mexico Idioma: En Revista: Appl Environ Microbiol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: França País de publicação: Estados Unidos