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Technological process for cell disruption, extraction and encapsulation of astaxanthin from Haematococcus pluvialis.
Machado, Francisco R S; Trevisol, Thalles C; Boschetto, Daiane L; Burkert, Janaína F M; Ferreira, Sandra R S; Oliveira, J Vladimir; Burkert, Carlos André V.
Afiliação
  • Machado FR; Bioprocess Engineering Laboratory, Chemistry and Food School, Federal University of Rio Grande, PO Box 474, 96203-900 Rio Grande, RS, Brazil.
  • Trevisol TC; Bioprocess Engineering Laboratory, Chemistry and Food School, Federal University of Rio Grande, PO Box 474, 96203-900 Rio Grande, RS, Brazil.
  • Boschetto DL; Department of Chemical and Food Engineering, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
  • Burkert JF; Bioprocess Engineering Laboratory, Chemistry and Food School, Federal University of Rio Grande, PO Box 474, 96203-900 Rio Grande, RS, Brazil.
  • Ferreira SR; Department of Chemical and Food Engineering, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
  • Oliveira JV; Department of Chemical and Food Engineering, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
  • Burkert CA; Bioprocess Engineering Laboratory, Chemistry and Food School, Federal University of Rio Grande, PO Box 474, 96203-900 Rio Grande, RS, Brazil. Electronic address: burkert@vetorial.net.
J Biotechnol ; 218: 108-14, 2016 Jan 20.
Article em En | MEDLINE | ID: mdl-26685712
In this work, the effectiveness of different enzymatic techniques for cell wall disruption of Haematococcus pluvialis for the extraction of carotenoids and subsequent encapsulation of extracts in the co-polymer poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) using the Solution Enhanced Dispersion by Supercritical fluids (SEDS) technique was investigated. Glucanex(®) performed best compared with Lyticase(®) and Driselase(®). The conditions for enzymatic lysis using this enzyme preparation were established as a pH of 4.5, a temperature of 55 °C, an initial activity of ß-1,3-glucanase of 0.6 U mL(-1) and a reaction time of 30 min. Enzymatic lysis assisted by ultrasound without biomass freezing was shown to be a promising and simple one-step technique for cell wall disruption, reaching 83.90% extractability. In the co-precipitation experiments, the highest encapsulation efficiency (51.21%) was obtained when using a higher biomass to dichloromethane ratio (10 mg mL(-1)) at the carotenoid extraction step and a lower pressure of precipitation (80 bar). In these conditions, spherical particles in the micrometer range (0.228 µm) were obtained.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Imobilizadas / Clorófitas Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Imobilizadas / Clorófitas Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda