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Effects of gemcitabine on radiosensitization, apoptosis, and Bcl-2 and Bax protein expression in human pancreatic cancer xenografts in nude mice.
Shen, Z T; Wu, X H; Wang, L; Li, B; Zhu, X X.
Afiliação
  • Shen ZT; Department of Radiation Oncology, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
  • Wu XH; Department of Radiation Oncology, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
  • Wang L; Department of Radiation Oncology, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
  • Li B; Department of Radiation Oncology, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
  • Zhu XX; Department of Radiation Oncology, Jinling Hospital, Medical School of Nanjing University, Nanjing, China xixuzhucn@163.com.
Genet Mol Res ; 14(4): 15587-96, 2015 Dec 02.
Article em En | MEDLINE | ID: mdl-26634526
The aim of this study was to evaluate the radiosensitizing effects of gemcitabine towards human pancreatic cancer xenografts. A human pancreatic cancer xenograft model was established in nude mice, 36 of which were randomly divided into 6 treatment groups. Tumors were measured every 2 days, and the tumor volumes, growth delays, and inhibition rates were compared to evaluate the gemcitabine enhancement factor. The apoptotic index was determined by terminal deoxynucleotidyl transferase dUTP nick end-labeling assay, and apoptosis inhibitory protein Bcl-2 and apoptosis-related protein Bax expression were detected by immunohistochemistry. Compared with the control group, xenograft growth was significantly inhibited in the 25 (G25) and 50 mg/kg gemcitabine (G50) groups (P < 0.05). In the 25 (G25R) and 50 (G50R) mg/kg gemcitabine + radiotherapy groups, local tumor growth was significantly inhibited, with inhibition rates of 88.22 and 91.23%, respectively, significantly higher than those of the simple radiotherapy (SR), G25, and G50 groups (44.11, 72.88, and 77.53%, respectively; P < 0.05). The tumor growth delay in the G25R and G50R groups were 9 and 15 days, respectively, higher than the SR, G25, and G50 groups (each 4 days, P < 0.05). The apoptosis of tumor cells in the intervention groups significantly increased, and the apoptotic index among the intervention groups exhibited significant differences (P < 0.05). The immunohistochemical results indicated that Bcl-2 was downregulated to different degrees in the intervention groups, whereas Bax was upregulated (P < 0.05). Therefore, gemcitabine appears to enhance the radiotherapeutic sensitivity of human pancreatic cancer xenografts significantly.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Pancreáticas / Tolerância a Radiação / Apoptose / Proteínas Proto-Oncogênicas c-bcl-2 / Desoxicitidina / Proteína X Associada a bcl-2 Tipo de estudo: Prognostic_studies Limite: Animals / Humans / Male Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Pancreáticas / Tolerância a Radiação / Apoptose / Proteínas Proto-Oncogênicas c-bcl-2 / Desoxicitidina / Proteína X Associada a bcl-2 Tipo de estudo: Prognostic_studies Limite: Animals / Humans / Male Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil